1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors Cystatin Family
  4. Cystatin D
  5. Cystatin D/CST5 蛋白, Human (HEK293, His)

Cystatin D/CST5 蛋白, Human (HEK293, His)

目录号: HY-P70020
产品使用指南

半胱氨酸蛋白酶抑制剂 D (CST5) 是一种半胱氨酸蛋白酶抑制剂,可预防口腔蛋白酶的侵害。它表现出特异性,优先抑制组织蛋白酶 S、组织蛋白酶 H、组织蛋白酶 L 和组织蛋白酶 B。这种微妙的调节强调了半胱氨酸蛋白酶抑制剂 D 在调节特定半胱氨酸蛋白酶中的作用,表明其在蛋白质稳态中的重要性以及对口腔健康的潜在参与。Cystatin D/CST5 蛋白, Human (HEK293, His) 是重组的 胱抑素 D/CST5 蛋白,由 HEK293 表达,带有 C-6*His 标签。Cystatin D/CST5 蛋白, Human (HEK293, His) 全长 122 个氨基酸,分子量约为 ~15.0 kDa。

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描述

Cystatin D (CST5), a cysteine proteinase inhibitor, potentially safeguards against oral cavity proteinases. Exhibiting specificity, it preferentially inhibits cathepsin S, cathepsin H, cathepsin L, and cathepsin B. This nuanced regulation emphasizes Cystatin D's role in modulating specific cysteine proteinases, signifying its importance in proteostasis and potential involvement in oral health. Cystatin D/CST5 Protein, Human (HEK293, His) is the recombinant human-derived Cystatin D/CST5 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cystatin D/CST5 Protein, Human (HEK293, His) is 122 a.a., with molecular weight of ~15.0 kDa.

研究背景

胱抑素 D (CST5) 是一种半胱氨酸蛋白酶抑制剂,据信对口腔中的蛋白酶具有潜在的保护功能。半胱氨酸蛋白酶抑制剂 D 的抑制活性具有特异性,对组织蛋白酶 S、组织蛋白酶 H、组织蛋白酶 L 和组织蛋白酶 B 的抑制具有优先顺序。这表明半胱氨酸蛋白酶抑制剂 D 在调节特定半胱氨酸蛋白酶的活性中具有微妙的调节作用,突出了其重要性维持蛋白质稳态并可能参与口腔健康。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P28325 (G21-V142)

基因 ID
蛋白结构
N-term
CST5 (G21-V142)
Accession # P28325
6*His
C-term
同用名
rHuCystatin-D/CST5, His; Cystatin-D; Cystatin-5; CST5
氨基酸序列

GSASAQSRTLAGGIHATDLNDKSVQCALDFAISEYNKVINKDEYYSRPLQVMAAYQQIVGGVNYYFNVKFGRTTCTKSQPNLDNCPFNDQPKLKEEEFCSFQINEVPWEDKISILNYKCRKV

分子量

Approximately 15.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cystatin D/CST5 Protein, Human (HEK293, His)
目录号:
HY-P70020
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