Dectin-1/CLEC7A 蛋白, Mouse (HEK293, N-His)

Dectin-1/CLEC7A acts as a lectin and specifically recognizes β-1,3-linked and β-1,6-linked glucans in bacterial and fungal cell walls. Dectin-1/CLEC7A is critical for Toll-like receptor 2 (TLR2)-mediated inflammation by recruiting SYK through its immunoreceptor tyrosine-based activation motif (ITAM), thereby activating NF-kappa-B. Dectin-1/CLEC7A Protein, Mouse (HEK293, N-His) is the recombinant mouse-derived Dectin-1/CLEC7A protein, expressed by HEK293 , with N-His labeled tag. The total length of Dectin-1/CLEC7A Protein, Mouse (HEK293, N-His) is 176 a.a., with molecular weight of ~25-30 kDa.

Dectin-1/CLEC7A 蛋白作为凝集素发挥作用，专门识别病原细菌和真菌细胞壁中发现的 β-1,3-连接和 β-1,6-连接葡聚糖。Dectin-1/CLEC7A 对于 Toll 样受体 2 (TLR2) 介导的炎症反应至关重要，Dectin-1/CLEC7A 通过其基于免疫受体酪氨酸的激活基序 (ITAM) 招募脾酪氨酸激酶 (SYK)，从而激活 NF-kappa-B。这启动了涉及 CARD 结构域-BCL10-MALT1 (CBM) 信号体的信号级联，导致 NF-kappa-B 和 MAP 激酶 p38 通路的激活。因此，这种级联刺激编码促炎细胞因子和趋化因子的基因的表达。此外，Dectin-1/CLEC7A 增强巨噬细胞和树突状细胞中细胞因子的产生，介导活性氧的产生，并促进白色念珠菌分生孢子的吞噬作用。值得注意的是，它独立于 T 细胞的表面聚糖而与 T 细胞结合，在 T 细胞激活、刺激 T 细胞增殖以及在 β-葡聚糖结合后诱导 SCIMP 磷酸化中发挥作用。该蛋白形成同二聚体并与 SYK 相互作用，有助于在真菌病原体存在的情况下激活白细胞。

Immobilized Mouse Dectin-1 at 2 μg/mL (100 μL/well) can bind Anti-Dectin-1 Antibody. The ED₅₀ for this effect is 0.6208 μg/mL.

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  Immobilized Mouse Dectin-1 at 2 μg/mL (100 μL/well) can bind Anti- Dectin-1 Antibody, The ED₅₀ for this effect is 0.6208 μg/mL.

Mouse

HEK293

N-His

NP_064392.2/Q6QLQ4 (F69-L244)

56644

FWRHNSGRNPEEKDNFLSRNKENHKPTESSLDEKVAPSKASQTTGGFSQPCLPNWIMHGKSCYLFSFSGNSWYGSKRHCSQLGAHLLKIDNSKEFEFIESQTSSHRINAFWIGLSRNQSEGPWFWEDGSAFFPNSFQVRNTAPQESLLHNCVWIHGSEVYNQICNTSSYSICEKEL

Approximately 25-30 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.