DENV2 Envelope 蛋白 (HEK293, His)

NS1 Protein, essential in virus budding, binds the cell membrane, forming a nucleocapsid that becomes the virus core. During entry, NS1 facilitates viral genome penetration post-hemifusion. NS1 migrates to the nucleus, influencing host functions, and counteracts host EXOC1's antiviral effects by sequestering and degrading EXOC1 via the proteasome pathway. Additionally, NS1 impedes RNA silencing by interfering with the host Dicer enzyme. DENV2 Envelope Protein (HEK293, His) is the recombinant DENV2 Envelope protein, expressed by HEK293, with N-His labeled tag.

NS1 蛋白在病毒出芽中起着至关重要的作用，因为它与细胞膜结合并将病毒 RNA 组装成核衣壳，核衣壳成为完全发育的病毒颗粒的核心。在病毒进入的过程中，NS1可能会在表面蛋白触发的半融合后促进病毒基因组渗透到宿主细胞质中。NS1 具有迁移至细胞核的能力，并在细胞核中对宿主功能产生影响。它可以通过蛋白酶体降解途径隔离和降解 EXOC1，抵消宿主 EXOC1 的抗病毒作用。此外，NS1 通过干扰宿主 Dicer 酶来阻碍 RNA 沉默。

Virus

HEK293

N-His

P29990 (M281-S677)

/

Partial

Approximately 55-60 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.