2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. DERP1 蛋白, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His)

DERP1 蛋白, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His)

目录号: HY-P75706
产品使用指南

DERP1 蛋白是一种硫醇蛋白酶，可选择性切割具有独特结构特征的目标蛋白，有利于 P2 位或碱性残基具有较大疏水侧链的底物。这种特异性强调了 DERP1 在细胞过程中的功能重要性。DERP1 蛋白, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His) 是重组的 DERP1 蛋白，由 HEK293 表达，带有 C-His 标签和 C132A，N150E 突变。DERP1 蛋白, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His) 全长 320 个氨基酸，分子量约为 ~35.7 kDa。

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
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100 μg   询价  

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描述

DERP1 Protein, a thiol protease, selectively cleaves target proteins with distinct structural features, favoring substrates with a large hydrophobic side chain in the P2 position or basic residues. This specificity underscores DERP1's functional significance in cellular processes. DERP1 Protein, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His) is the recombinant DERP1 protein, expressed by HEK293 , with C-His labeled tag and C132A, N150E mutation. The total length of DERP1 Protein, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His) is 320 a.a., with molecular weight of ~35.7 kDa.
研究背景

DERP1 蛋白被鉴定为硫醇蛋白酶，对 P2 位有大疏水侧链或含有碱性残基的底物表现出明显的偏好。这种特异性强调了它在选择性切割具有特定结构特征的靶蛋白方面的作用，突出了该酶在细胞过程中的功能意义。
种属

Others
表达系统

HEK293
标签

C-His
蛋白编号

P08176 (M1-L320, C132A, N150E)
基因 ID

113791973  [NCBI]
蛋白结构
N-term
DERP1 (M1-L320, C132A, N150E)
Accession # P08176
His
C-term
同用名
Peptidase 1; Allergen Der p I; DERP1
分子量

Approximately 35.7 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DERP1 Protein, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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