2. 重组蛋白
  3. Enzymes & Regulators
  4. Phosphatase
  5. Protein tyrosine phosphatases
  6. Dual Specificity Phosphatase 3 (DUSP3)
  7. DUSP3 蛋白, Human (His)

DUSP3 蛋白, Human (His)

目录号: HY-P71150
COA 产品使用指南

DUSP3 是一种双特异性磷酸酶，优先使磷酸酪氨酸去磷酸化。其主要功能包括灭活 ERK1 和 ERK2，从而调节相关的细胞信号通路。DUSP3 蛋白, Human (His) 是重组的 DUSP3 蛋白，由 E. coli 表达，带有 N-6*His 标签。DUSP3 蛋白, Human (His) 全长 184 个氨基酸，分子量为 18-22 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μg ￥960 In-stock
50 μg ￥2700 In-stock
100 μg   询价  

* Please select Quantity before adding items.

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描述

DUSP3, a dual-specificity phosphatase, preferentially dephosphorylates phosphotyrosines. Its main function involves inactivating ERK1 and ERK2, thereby modulating associated cellular signaling pathways. DUSP3 Protein, Human (His) is the recombinant human-derived DUSP3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of DUSP3 Protein, Human (His) is 184 a.a., with molecular weight of 18-22 kDa.
研究背景

DUSP3 蛋白表现出双重磷酸酶活性，同时靶向酪氨酸蛋白磷酸盐和丝氨酸蛋白磷酸盐，并且对磷酸酪氨酸具有显着的偏好。其主要功能涉及 ERK1 和 ERK2 的去磷酸化和随后的失活，从而调节与这些激酶相关的细胞信号传导途径。
生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
种属

Human
表达系统

E. coli
标签

N-6*His
蛋白编号

P51452 (S2-P185)
基因 ID
蛋白结构
N-term
6*His
DUSP3 (S2-P185)
Accession # P51452
C-term
同用名
Dual specificity protein phosphatase 3; DUSP3; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR
氨基酸序列

SGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNASVAQDIPKLQKLGITHVLNAAEGRSFMHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGRVLVHCREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDRLAKEGKLKP

分子量

18-22 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液
组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
内毒素含量

<1 EU/μg, determined by LAL method.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (208 KB)

产品使用指南 (1213 KB)

DUSP3 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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目录号：
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