2. 重组蛋白
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  4. DUSP3 蛋白, Human (sf9)

DUSP3 是一种双特异性磷酸酶，优先使磷酸酪氨酸去磷酸化。其主要功能包括灭活 ERK1 和 ERK2，从而调节相关的细胞信号通路。DUSP3 蛋白, Human (sf9) 是重组的 DUSP3 蛋白，由 Sf9 insect cells 表达，不带标签。

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     可免费申领三个不同产品的试用装。

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描述

DUSP3, a dual-specificity phosphatase, preferentially dephosphorylates phosphotyrosines. Its main function involves inactivating ERK1 and ERK2, thereby modulating associated cellular signaling pathways. DUSP3 Protein, Human (sf9) is the recombinant human-derived DUSP3 protein, expressed by Sf9 insect cells, with tag free.
研究背景

DUSP3 蛋白表现出双重磷酸酶活性，同时靶向酪氨酸蛋白磷酸盐和丝氨酸蛋白磷酸盐，并且对磷酸酪氨酸具有显着的偏好。其主要功能涉及 ERK1 和 ERK2 的去磷酸化和随后的失活，从而调节与这些激酶相关的细胞信号传导途径。
种属

Human
表达系统

Sf9 insect cells
标签

Tag Free
蛋白编号

P51452-1 (N-G&P, M1-P185)
基因 ID

1845
蛋白长度

Full Length
中文名
D泛素特异性肽酶 3 蛋白
同用名
Dual specificity protein phosphatase 3; DUSP3; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR
预测分子量
20.6 kDa
分子量

Approximately 21 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DUSP3 Protein, Human (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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