1. 重组蛋白
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. ECH1 蛋白, Human (His)

ECH1 蛋白, Human (His)

目录号: HY-P70059
产品使用指南

ECH1 蛋白在异构化过程中发挥着至关重要的作用,可将 3-反式,5-顺式二烯酰辅酶A 转化为其异构形式 2-反式,4-反式二烯酰辅酶A。这一酶促步骤在代谢途径中至关重要,可确保二烯酰辅酶 A 物种的平衡并促进整体代谢通量。ECH1 的活性对于维持辅酶 A (CoA) 连接中间体的结构重排至关重要,强调了其在细胞过程中的重要性。ECH1 蛋白, Human (His) 是重组的 ECH1 蛋白,由 E. coli 表达,带有 N-6*His 标签。ECH1 蛋白, Human (His) 全长 295 个氨基酸,分子量为 30-35 kDa。

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描述

ECH1 Protein plays a crucial role in isomerization, converting 3-trans,5-cis-dienoyl-CoA to its isomeric form, 2-trans,4-trans-dienoyl-CoA. This enzymatic step is vital in metabolic pathways, ensuring the equilibrium of dienoyl-CoA species and contributing to overall metabolic flux. ECH1's activity is essential for maintaining structural rearrangement in Coenzyme A (CoA)-linked intermediates, emphasizing its significance in cellular processes. ECH1 Protein, Human (His) is the recombinant human-derived ECH1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ECH1 Protein, Human (His) is 295 a.a., with molecular weight of 30-35 kDa.

研究背景

ECH1 在异构化酶促过程中发挥着至关重要的作用,具体是将 3-反式,5-顺式-二烯酰基-CoA 转化为其异构形式 2-反式,4-反式-二烯酰基-CoA。该异构化步骤对于辅酶 A (CoA) 连接的中间体进行结构重排的各种代谢途径至关重要。ECH1 催化这种异构体转化的能力有助于整体代谢通量,突出了其在维持细胞过程中二烯酰辅酶 A 物种平衡方面的重要性。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q13011 (T34-L328)

基因 ID
蛋白结构
N-term
6*His
ECH1 (T34-L328)
Accession # Q13011
C-term
同用名
rHuDelta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial/ECH1, His; Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase; mitochondrial; ECH1;
氨基酸序列

TGSSAQEEASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

分子量

30-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ECH1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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