EEF1A1 蛋白, Human (His-SUMO)

EEF1A1 is a translation elongation factor that catalyzes GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the ribosomal A site during protein synthesis. This involves mRNA codon-aa-tRNA anticodon base pairing, leading to GTP hydrolysis and aa-tRNA release in EEF1A1. EEF1A1 Protein, Human (His-SUMO) is the recombinant human-derived EEF1A1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of EEF1A1 Protein, Human (His-SUMO) is 462 a.a., with molecular weight of ~66.1 kDa.

EEF1A1 是一种翻译延伸因子，在延伸阶段催化氨酰基-tRNA (aa-tRNA) 与核糖体 A 位点的 GTP 依赖性结合，从而在蛋白质合成中发挥至关重要的作用。该过程涉及 mRNA 密码子和 aa-tRNA 反密码子之间的碱基配对，导致 GTP 水解并随后从 EEF1A1 中释放 aa-tRNA。然后，释放的 aa-tRNA 被容纳到核糖体中，通过核糖体催化的肽键形成促进不断增长的蛋白质链的延伸。除了其核心翻译功能外，EEF1A1 还参与 T 辅助细胞 1 细胞中 IFNG 转录的正向调节，与 TXK 和 PARP1 形成 IFNG 启动子结合复合物。此外，在微生物感染的情况下，EEF1A1对于病毒蛋白的翻译和人类冠状病毒SARS-CoV-2的复制至关重要，突显了其在细胞和致病翻译过程中的关键作用。

Human

E. coli

N-6*His;N-SUMO

P68104 (M1-K462)

1915  [NCBI]

MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK

Approximately 66.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.