EGLN1 蛋白, Human (His)

EGLN1 Protein, Human (His) is the recombinant human-derived EGLN1, expressed by E. coli , with His labeled tag.

EGLN1 是一种细胞氧传感器，在常氧条件下催化缺氧诱导因子 (HIF) α 蛋白中 4-羟基脯氨酸的翻译后形成。它能羟基化 HIF1A 的每个氧依赖性降解域 (ODD) 中的特定脯氨酸残基 (N 端 NODD 和 C 端 CODD)，并对 HIF2A 也有类似作用。EGLN1 对 HIF1A 和 HIF1B 的 CODD 位点具有偏好性。羟基化的 HIF 随后通过 von Hippel-Lindau 泛素化复合体靶向蛋白酶体降解。在缺氧条件下，羟基化反应减弱，使 HIF 逃逸降解，进而转位至细胞核，与 HIF1B 形成异源二聚体，并增强缺氧诱导基因的表达。EGLN1 是常氧下最重要的同工酶，通过调节 HIF1 的稳定性参与多种缺氧相关过程，如视网膜和心脏功能中的血管生成。其靶蛋白优先通过 LXXLAP 基序被识别。

Human

E. coli

N-His

Q9GZT9-1 (P181-F426)

54583

Partial

28.4 KDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.22 μm filtered solution of 25mM Tris-HCl (pH 7.5), 150mM NaCl.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.