Eotaxin/CCL11 蛋白, Mouse

Eotaxin/CCL11 Protein, Mouse is a potent chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia.

CCL11 是属于 CC 趋化因子家族的一种小细胞因子。CCL11 通过诱导嗜酸性粒细胞的趋化性选择性地募集嗜酸性粒细胞，因此与过敏反应有关^[1]。它由 IFN-γ 刺激的内皮细胞和 TNF 激活的单核细胞产生。CCL11 是嗜酸性粒细胞、嗜碱性粒细胞和 Th2 淋巴细胞的有效化学引诱剂。CCL11 在疾病过程中具有许多重要的生物学功能。它在过敏性和非过敏性炎症反应（例如分枝杆菌和血吸虫引起的肉芽肿病）中发挥着关键作用^[2]。

In CCL11^-/- BALB/c mice, methylcholanthrene (MCA) at a concentration of 100 μg can induce fibrosarcoma in 90% of mice, possibly associated with a reduction in eosinophil influx into the tumor, which directly kills MCA-induced fibrosarcoma cells^[2].
       CCL11 expression is produced in the peritoneum, serum and lung and peaked after 4 h  intraperitoneal injection of 500 μg lipopolysaccharide (LPS) in 1 mL saline in murine model of sublethal endotoxemia. When eotaxin/CCL11 knockout mice are treated with LPS, there is an increase in peritoneal neutrophils, but not lung neutrophils, compared to wild-type controls. CCL11^-/- mice suppresses peritoneal neutrophils associated with endotoxemia^[3].

1.Full biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/mL.
2.Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is ≤2.06 ng/mL, corresponding to a specific activity is ≥4.854×10⁵ units/mg.

• 
  Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 1.804 ng/mL, corresponding to a specific activity is 5.543×10⁵ units/mg.

Mouse

E. coli

Tag Free

P48298 (H24-P97)

20292  [NCBI]

HPGSIPTSCCFIMTSKKIPNTLLKSYKRITNNRCTLKAIVFKTRLGKEICADPKKKWVQDATKHLDQKLQTPKP

Approximately 8-13.28 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Antonio L. Teixeira, et al. Revisiting the Role of Eotaxin-1/CCL11 in Psychiatric Disorders. Front Psychiatry. 2018 Jun 14;9:241.  [Content Brief]

  [2]. Garcia-Zepeda EA, et al. Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat Med. 1996 Apr;2(4):449-56.  [Content Brief]

  [3]. R Salcedo, et al. Eotaxin (CCL11) induces in vivo angiogenic responses by human CCR3+ endothelial cells. J Immunol. 2001 Jun 15;166(12):7571-8.  [Content Brief]

  [4]. Salcedo R, et al. Eotaxin (CCL11) induces in vivo angiogenic responses by human CCR3+ endothelial cells. J Immunol. 2001 Jun 15;166(12):7571-8.  [Content Brief]

  [5]. Ljubov Simson, et al. Regulation of carcinogenesis by IL-5 and CCL11: a potential role for eosinophils in tumor immune surveillance. J Immunol. 2007 Apr 1;178(7):4222-9.  [Content Brief]