1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins
  3. Ephrin/Eph Family Cytokine Receptors
  4. Ephrins
  5. Ephrin-A5
  6. Ephrin-A5/EFNA5 蛋白, Rat (HEK293, His)

Ephrin-A5/EFNA5 蛋白, Rat (HEK293, His)

目录号: HY-P73014
COA 产品使用指南

Ephrin-A5/EFNA5 蛋白是一种重要的细胞表面配体,在神经元、血管和上皮发育中发挥重要作用。它与 Eph 受体结合,启动双向信号传导并调节细胞间粘附、细胞骨架组织和晶状体透明度。Ephrin-A5/EFNA5 蛋白, Rat (HEK293, His) 是重组的 Ephrin-A5/EFNA5 蛋白,由 HEK293 表达,带有 C-His 标签。Ephrin-A5/EFNA5 蛋白, Rat (HEK293, His) 全长 182 个氨基酸,分子量约为 ~27 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥350 In-stock
10 μg ¥600 In-stock
50 μg ¥1650 In-stock
100 μg ¥2800 In-stock
500 μg ¥7850 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The Ephrin-A5/EFNA5 protein is an important cell surface ligand that plays an important role in neuronal, vascular, and epithelial development. It binds to Eph receptors, initiating bidirectional signaling and regulating intercellular adhesion, cytoskeletal organization, and lens transparency. Ephrin-A5/EFNA5 Protein, Rat (HEK293, His) is the recombinant rat-derived Ephrin-A5/EFNA5 protein, expressed by HEK293 , with C-His labeled tag. The total length of Ephrin-A5/EFNA5 Protein, Rat (HEK293, His) is 182 a.a., with molecular weight of ~27 kDa.

研究背景

Ephrin-A5/EFNA5 蛋白是 Eph 受体的细胞表面 GPI 结合配体,在神经元、血管和上皮发育中发挥着至关重要的作用,其中 Eph 受体对于迁移、排斥和粘附至关重要。EFNA5 与相邻细胞上的 Eph 受体混杂结合,启动接触依赖性双向信号传导,其中正向信号传导在受体下游,反向信号传导在肝配蛋白配体下游。当与其同源受体结合时,EFNA5 会在小窝样膜微域内诱导区室化信号传导,需要 Fyn 酪氨酸激酶的活性。它激活 EPHA3 受体来调节细胞间粘附和细胞骨架组织,并与 EPHA2 结合,可能在塑造晶状体纤维细胞和维持晶状体透明度方面发挥作用。此外,EFNA5 刺激轴突束颤并介导胰岛细胞之间的通讯,通过与 EPHA5 的相互作用调节葡萄糖刺激的胰岛素分泌。它也是 EPHA7 的同源配体,通过调节细胞间粘附和排斥来影响大脑发育。此外,EFNA5 与 EPHA8 相互作用,激活该受体,并与 EPHA3ADAM10 形成三元复合物,介导细胞外结构域脱落并调节 EFNA5-EPHA3 复合物的内化和功能。

生物活性

Immobilized rat EFNA5 at 10 μg/mL (100 μL/well) can bind Mouse EPHA3. The ED50 for this effect is 150.5 ng/mL.

  • Immobilized rat EFNA5 at 10 μg/ml (100 μl/well) can bind Mouse EPHA3. The ED50 for this effect is 150.5 ng/mL.
种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P97605 (Q21-E202)

基因 ID

116683  [NCBI]

蛋白结构
N-term
EFNA5 (Q21-E202)
Accession # P97605
His
C-term
同用名
Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7; EFNA5; EPLG7
氨基酸序列

QDPGSKVVADRYAVYWNSSNPRFQRGDYHIDVCINDYLDVFCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVRDRVFDVNDKVENSLEPADDTVHESAEPSRGE

分子量

Approximately 27 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Ephrin-A5/EFNA5 Protein, Rat (HEK293, His)
目录号:
HY-P73014
需求量: