1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Epiregulin
  5. Epiregulin 蛋白, Human

Epiregulin 蛋白, Human

目录号: HY-P7011
COA 产品使用指南

Epiregulin 蛋白, Human是一种生长调节剂,是表皮生长因子 (EGF) 家族的成员。是一种肿瘤生长抑制因子,可诱导人表皮样癌 HeLa 细胞的形态变化。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥350 In-stock
10 μg ¥950 In-stock
50 μg ¥2650 In-stock
100 μg ¥4500 In-stock
500 μg ¥12000 询价
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Epiregulin Protein, Human is a growth regulator and a member of the epidermal growth factor (EGF) family. Epiregulin is a tumor growth-inhibitory factor inducing morphological changes in human epidermoid carcinoma HeLa cells.

研究背景

人类上皮调节蛋白基因编码一个由163个残基组成的推定跨膜前体,其内部片段含有一个EGF样结构域,其结构组织与与EGF受体结合的EGF家族其他成员相似。上皮调节蛋白具有不同于 EGF 家族经典成员、EGF 和转化生长因子 α 的某些特征,包括对几种正常细胞的促有丝分裂反应以及与表皮样癌 A431 细胞上的 EGF 受体结合。Northern blot分析显示,人上皮调节蛋白主要在正常组织的外周血巨噬细胞和胎盘上表达,在各类肿瘤细胞系中,在上皮性肿瘤细胞系上表达最高。上皮调节蛋白参与某些生理过程,例如正常细胞生长的维持或发展以及癌症的进展[1]

生物活性

1. The ED50 is <2 ng/mL as measured by murine Ballb/c 3T3 cells, corresponding to a specific activity of >5 × 105 units/mg.
2. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.3417 ng/mL, corresponding to a specific activity is 2.927×10^6 units/mg.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

O14944 (V60-L108)

基因 ID
蛋白结构
N-term
Epiregulin (V60-L108)
Accession # O14944
C-term
同用名
rHuEpiregulin; EREG; EPR
氨基酸序列

VAQVSITKCSSDMNGYCLHGQCIYLVDMSQNYCRCEVGYTGVRCEHFFL

分子量

Approximately 5.6 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Epiregulin Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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