ERVW-1 蛋白, Human (His-SUMO)

The ERVW-1 protein is an endogenous retroviral envelope protein that retains fusogenic properties critical for trophoblast fusion and syncytium formation during placental morphogenesis. Its fusion ability involves binding to SLC1A4 and SLC1A5. ERVW-1 Protein, Human (His-SUMO) is the recombinant human-derived ERVW-1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of ERVW-1 Protein, Human (His-SUMO) is 423 a.a., with molecular weight of ~63.0 kDa.

内源性逆转录病毒包膜蛋白 ERVW-1 保留了其固有的融合特性，在胎盘形态发生过程中滋养层融合和合胞体的建立中发挥着关键作用。通过 SLC1A4 和 SLC1A5 的结合促进其诱导融合的能力。内源性包膜蛋白，如 ERVW-1，可能表现出不同的进化命运，随着时间的推移维持、丢失或改变其原始功能。逆转录病毒包膜蛋白包括用于受体识别的表面蛋白（SU）和作为I类病毒融合蛋白的跨膜蛋白（TM），对于早期感染中的受体识别和膜融合至关重要。ERVW-1可能采用三种构象状态：融合前天然状态、发夹前中间状态和融合后发夹状态。在病毒和靶细胞膜融合的情况下，卷曲螺旋区域（七肽重复序列）转变为发夹三聚体结构，使融合肽靠近胞外域的 C 末端区域——这种结构排列被认为可以驱动膜并置以及随后的融合。

Human

E. coli

N-His;N-SUMO

Q9UQF0 (21A-443Q)

30816  [NCBI]

APPPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTHMPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQAREKHVKEVISQLTRVHGTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTNCWICLPLNFRPYVSIPVPEQWNNFSTEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTTYTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCLNGSSESMCFLSFLVPPMTIYTEQDLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKLSQELNGDMERVADSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKEIRDRIQRRAEELRNTGPWGLLSQ

Approximately 63.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.