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  3. Erythropoietin receptor/EpoR 蛋白, Human (226a.a, HEK293, His)

Erythropoietin receptor/EpoR 蛋白, Human (226a.a, HEK293, His)

目录号: HY-P70314
COA 产品使用指南

EPOR 蛋白作为促红细胞生成素受体发挥作用,在 EPO 刺激下介导成红细胞增殖和分化。异二聚体触发 JAK2/STAT5 信号级联,并在某些细胞类型中激活 STAT1、STAT3 和 LYN 酪氨酸激酶。此外,异构体 EPOR-T 作为显性失活受体,调节 EPOR 介导的信号通路。Erythropoietin receptor/EpoR 蛋白, Human (226a.a, HEK293, His) 是重组的 促红细胞生成素受体/EpoR 蛋白,由 HEK293 表达,带有 C-6*His 标签。Erythropoietin receptor/EpoR 蛋白, Human (226a.a, HEK293, His) 全长 226 个氨基酸,分子量约为 approximately 32.54 kDa。

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描述

The EPOR Protein functions as a receptor for erythropoietin, mediating erythroblast proliferation and differentiation upon EPO stimulation. The heterodimer triggers the JAK2/STAT5 signaling cascade and, in certain cell types, activates STAT1, STAT3, and the LYN tyrosine kinase. Additionally, isoform EPOR-T acts as a dominant-negative receptor, modulating EPOR-mediated signaling pathways. Erythropoietin receptor/EpoR Protein, Human (226a.a, HEK293, His) is the recombinant human-derived Erythropoietin receptor/EpoR protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Erythropoietin receptor/EpoR Protein, Human (226a.a, HEK293, His) is 226 a.a., with molecular weight of approximately 32.54 kDa.

研究背景

EPOR 作为促红细胞生成素 (EPO) 的受体,在介导 EPO 诱导的成红细胞增殖和分化中发挥着至关重要的作用。受到 EPO 刺激后,异源二聚体的 EPOR 成分发生二聚化,启动 JAK2/STAT5 信号级联反应。在某些细胞类型中,这种异二聚体受体复合物还可以激活 STAT1STAT3,并可能参与 LYN 酪氨酸激酶的激活。值得注意的是,同种型 EPOR-T 作为显性失活受体,调节和减弱 EPOR 介导的信号通路。EPORic 复合物内错综复杂的相互作用强调了其在调节细胞对 EPO 刺激反应中的重要性。

生物活性

Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 79.98 ng/mL, corresponding to a specific activity is 1.2053×104 units/mg.

  • Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 79.98 ng/mL, corresponding to a specific activity is 1.2053×104 units/mg.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P19235 (A25-P250)

基因 ID
蛋白结构
N-term
EpoR (A25-P250)
Accession # P19235
6*His
C-term
同用名
rHuErythropoietin receptor/EpoR, His; EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358
氨基酸序列

APPPNLPDPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGNYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGAPRYHRVIHINEVVLLDAPVGLVARLADESGHVVLRWLPPPETPMTSHIRYEVDVSAGNGAGSVQRVEILEGRTECVLSNLRGRTRYTFAVRARMAEPSFGGFWSAWSEPVSLLTPSDLDP

分子量

approximately 32.54 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Erythropoietin receptor/EpoR Protein, Human (226a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Erythropoietin receptor/EpoR Protein, Human (226a.a, HEK293, His)
目录号:
HY-P70314
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