1. 重组蛋白
  2. Cytokines and Growth Factors
  3. FGF Family
  4. FGFBP3 蛋白, Human (sf9, His)

FGFBP3 蛋白, Human (sf9, His)

目录号: HY-P76926
产品使用指南

FGFBP3 蛋白是一种肝素结合蛋白,可复杂地调节成纤维细胞生长因子 2 (FGF2) 动态。通过与 FGF2 形成结合复合物,它可阻断 FGF2 的肝素结合,并可能限制其锚定在细胞外基质糖胺聚糖上。FGFBP3 蛋白, Human (sf9, His) 是重组的 FGFBP3 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。FGFBP3 蛋白, Human (sf9, His) 全长 258 个氨基酸,分子量约为 ~33 kDa。

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描述

The FGFBP3 protein is a heparin-binding protein that complexly regulates fibroblast growth factor 2 (FGF2) dynamics. By forming a binding complex with FGF2, it blocks heparin binding of FGF2 and may limit its anchoring to extracellular matrix glycosaminoglycans. FGFBP3 Protein, Human (sf9, His) is the recombinant human-derived FGFBP3 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FGFBP3 Protein, Human (sf9, His) is 258 a.a., with molecular weight of ~33 kDa.

研究背景

FGFBP3 蛋白被鉴定为肝素结合蛋白,复杂地调节成纤维细胞生长因子 2 (FGF2) 的动态。这种多功能蛋白质与 FGF2 形成结合复合物,同时阻碍其与肝素的结合,并可能阻碍 FGF2 在细胞外基质糖胺聚糖上的固定。这种双重机制促进 FGF2 的释放,使其释放并随后启动成纤维细胞生长因子受体 (FGFR) 信号传导。由 FGFBP3 蛋白协调的 FGFR 信号下游激活与血管通透性的增强有关。重要的是,FGFBP3 蛋白和 FGF2 之间的直接相互作用强调了该蛋白在协调这些复杂的细胞过程中的关键作用。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q8TAT2 (M1-G258)

基因 ID
蛋白结构
N-term
FGFBP3 (M1-G258)
Accession # Q8TAT2
His
C-term
同用名
Fibroblast growth factor-binding protein 3; FGF-BP3; C10orf13
分子量

Approximately 33 kDa

纯度

Greater than 87% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FGFBP3 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FGFBP3 Protein, Human (sf9, His)
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HY-P76926
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