1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Epithelial cell CD Proteins G-Protein-Coupled Receptors (GPCRs)
  4. Frizzled-10/CD350 Frizzled
  5. Frizzled-10
  6. Frizzled-10/CD350 蛋白, Mouse (HEK293, His)

Frizzled-10/CD350 蛋白, Mouse (HEK293, His)

目录号: HY-P74144
产品使用指南

Frizzled-10/CD350 蛋白是 Wnt 蛋白的受体,在经典 Wnt/β-catenin 通路中发挥作用,激活蓬乱的蛋白,抑制 GSK-3 激酶,并触发 Wnt 靶基因激活。次要信号通路涉及 PKC 和钙通量。Frizzled-10/CD350 蛋白, Mouse (HEK293, His) 是重组的 Frizzled-10/CD350 蛋白,由 HEK293 表达,带有 C-His 标签。Frizzled-10/CD350 蛋白, Mouse (HEK293, His) 全长 141 个氨基酸,分子量约为 ~23 kDa。

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描述

Frizzled-10/CD350 protein is a receptor for Wnt proteins that functions in the canonical Wnt/β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target gene activation. Secondary signaling pathways involve PKC and calcium flux. Frizzled-10/CD350 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Frizzled-10/CD350 protein, expressed by HEK293 , with C-His labeled tag. The total length of Frizzled-10/CD350 Protein, Mouse (HEK293, His) is 141 a.a., with molecular weight of ~23 kDa.

研究背景

Frizzled-10/CD350 蛋白作为 Wnt 蛋白的受体,主要在经典 Wnt/β-连环蛋白信号通路中发挥作用。该途径协调混乱蛋白质的激活,抑制 GSK-3 激酶,促进 β-连环蛋白的核积累,并触发 Wnt 靶基因的激活。除了经典途径外,一些家族成员还发现了涉及 PKC 和钙通量的次级信号传导途径。鉴于 PKCWnt 介导的 GSK-3 激酶失活中的明显作用,这些途径及其潜在整合之间的相互作用仍有待充分阐明。Frizzled-10 可能在组织形态发生期间或分化组织内的极性信息转导和传递中发挥至关重要的作用。与 MYOC 和 WNT7B 的相互作用强调了它参与复杂的细胞过程和信号转导级联。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q8BKG4/NP_780493.1(I22-G162)

基因 ID

93897  [NCBI]

蛋白结构
N-term
CD350 (I22-G162)
Accession # Q8BKG4/NP_780493.1
His
C-term
同用名
CD350 antigen; CD350; Frizzled-10; FZ-10; FZD10
分子量

Approximately 23 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Frizzled-10/CD350 Protein, Mouse (HEK293, His)
目录号:
HY-P74144
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