GABARAPL1/GEC-1 蛋白, Human (His)

GABARAPL1/GEC-1 is a multifunctional ubiquitin-like modifier that contributes to cell surface expression of kappa-type opioid receptors and contributes to autophagy, shaping autophagosome vacuoles. Unlike LC3, which is involved in phagophore elongation, the GABARAP/GATE-16 subfamily plays a crucial role in late autophagosome maturation. GABARAPL1/GEC-1 Protein, Human (His) is the recombinant human-derived GABARAPL1/GEC-1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GABARAPL1/GEC-1 Protein, Human (His) is 117 a.a., with molecular weight of ~20.0 kDa.

GABARAPL1/GEC-1 是一种泛素样修饰剂，在细胞过程中发挥多方面的作用。它通过促进 kappa 型阿片受体的顺行细胞内运输来增强其细胞表面表达。此外，GABARAPL1/GEC-1 有助于自噬，参与自噬体液泡的形成。虽然 LC3 与吞噬细胞膜伸长相关，但 GABARAP/GATE-16 亚家族在自噬体成熟的后期阶段发挥着至关重要的作用。此外，GABARAPL1/GEC-1与网状吞噬受体TEX264合作，在营养应激期间将内质网的子结构域重塑为自噬体，促进随后与溶酶体的融合以实现内质网周转。该蛋白参与与各种伙伴的相互作用网络，包括 ATG13、OPRK1、RB1CC1、ULK1、TP53INP1、TP53INP2、SQSTM1、ATG3、ATG7、MAP15、TECPR2、TBC1D5、MAPK15、TRIM5、MEFV、TRIM21、WDFY3、UBA5、KBBTD6 、KBTBD7、RETREG1、RETREG2、RETREG3、TEX264 和 IRGM，强调了其在细胞稳态和自噬过程中的重要性。

Human

E. coli

N-6*His

Q9H0R8 (M1-K117)

23710  [NCBI]

MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK

Approximately 20.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of sterile 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.