GDF-15 蛋白, Rat (His)

GDF-15 Protein regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stresses. Binding to its receptor, GFRAL, activates GFRAL-expressing neurons in the brainstem, triggering a 'stress response circuit.' This involves neurons in the parabrachial nucleus and central amygdala, shaping feeding responses during stress. Additionally, GDF-15 inhibits growth hormone signaling on hepatocytes. Existing as a disulfide-linked homodimer, it acts as a ligand for GFRAL, mediating GDF15 internalization and cellular signaling through RET. GDF-15 Protein, Rat (His) is the recombinant rat-derived GDF-15 protein, expressed by E. coli, with N-His labeled tag. The total length of GDF-15 Protein, Rat (His) is 115 a.a., with molecular weight of 15-17 kDa.

GDF-15 蛋白在调节食物摄入、能量消耗和体重以应对代谢和毒素引起的压力方面发挥着至关重要的作用。它与其受体 GFRAL 结合，导致位于脑干后区和孤束核的表达 GFRAL 的神经元激活。这种激活随后触发臂旁核和杏仁核中央神经元的激活，形成“紧急回路”的一部分，参与在压力条件下塑造进食反应。此外，GDF-15 蛋白还可抑制肝细胞上的生长激素信号传导。它以二硫键连接的同二聚体形式存在，并与作为其配体的 GFRAL 相互作用，通过与 RET 相互作用介导 GDF15 内化和细胞信号传导。

Immobilized Rat GDF15, His Tag at 5 μg/mL (100 μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC₅₀ of <6.2 μg/mL determined by ELISA.

Rat

E. coli

N-His

Q9Z0J6 (S189-A303)

29455  [NCBI]

SAHLHPRDSCPLGPGRCCHLETVQATLEDLGWSDWVLSPRQLQLSMCVGECPHLYRSANTHALIKARLHGLQPDRVPAPCCVPSSYTPVVLMHRTDSGVSLQTYDDLVAQGCHCA

15-17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50mM HAc, pH 2.9. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 50mM HAc, pH 2.9.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.