1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. GLUD1 蛋白, Human (His)

GLUD1 蛋白, Human (His)

目录号: HY-P701915
产品使用指南

GLUD1 蛋白是一种线粒体谷氨酸脱氢酶,可将 L-谷氨酸转化为 α-酮戊二酸,支持谷氨酰胺回补和三羧酸循环。除了代谢之外,GLUD1 在胰岛素稳态中也发挥着关键作用,复杂地调节葡萄糖代谢。GLUD1 蛋白, Human (His) 是重组的 GLUD1 蛋白,由 E. coli 表达,带有 N-6*His 标签。GLUD1 蛋白, Human (His) 全长 505 个氨基酸。

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描述

GLUD1 protein is a mitochondrial glutamate dehydrogenase that converts L-glutamate into α-ketoglutarate, supporting glutamine anaplerosis and the tricarboxylic acid cycle. In addition to metabolism, GLUD1 plays a key role in insulin homeostasis, complexly regulating glucose metabolism. GLUD1 Protein, Human (His) is the recombinant human-derived GLUD1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GLUD1 Protein, Human (His) is 505 a.a., .

研究背景

GLUD1 蛋白是一种线粒体谷氨酸脱氢酶,是 L-谷氨酸转化为 α-酮戊二酸的关键催化剂,从而在谷氨酰胺回补中发挥关键作用,并为三羧酸循环提供关键的中间体。除了其代谢功能之外,GLUD1 在胰岛素稳态中发挥着关键作用,有助于复杂的葡萄糖代谢调节。此外,有暗示性证据表明 GLUD1 参与认知过程,暗示其通过调节兴奋性神经递质谷氨酸周转可能参与学习和记忆反应。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P00367 (S54-T558)

基因 ID

2746

蛋白结构
N-term
6*His
GLUD1 (S54-T558)
Accession # P00367
C-term
同用名
GLUD1; Glutamate dehydrogenase 1; mitochondrial; GDH 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GLUD1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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