1. 重组蛋白
  2. CAR-T related Proteins
  3. GPC-3
  4. Glypican-3/GPC3 蛋白, Human (HEK293, Fc)

Glypican-3/GPC3 蛋白, Human (HEK293, Fc)

目录号: HY-P70367
COA 产品使用指南

Glypican-3 (GPC3) 蛋白是一种细胞表面蛋白聚糖,在对发育过程至关重要的关键信号传导通路中发挥复杂的调节作用。通过其 GPI 锚定,GPC3 通过与 Hedgehog 受体 PTC1 竞争与 Hedgehog 蛋白的结合,负向调节 Hedgehog 信号通路,导致复杂的内化和随后的溶酶体降解。Glypican-3/GPC3 蛋白, Human (HEK293, Fc) 是重组的 Glypican-3/GPC3 蛋白,由 HEK293 表达,带有 C-hFc 标签。Glypican-3/GPC3 蛋白, Human (HEK293, Fc) 全长 535 个氨基酸,分子量分别为 100-120 & 65 & 40 kDa。

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规格 价格 是否有货 数量
10 μg ¥650 In-stock
50 μg ¥1800 In-stock
100 μg   询价  

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描述

The Glypican-3 (GPC3) protein is a cell surface proteoglycan that plays complex regulatory roles in key signaling pathways critical to developmental processes. Through its GPI anchoring, GPC3 negatively regulates the Hedgehog signaling pathway by competing with the Hedgehog receptor PTC1 for binding to Hedgehog protein, leading to complex internalization and subsequent lysosomal degradation. Glypican-3/GPC3 Protein, Human (HEK293, Fc) is the recombinant human-derived Glypican-3/GPC3 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Glypican-3/GPC3 Protein, Human (HEK293, Fc) is 535 a.a., with molecular weight of 100-120 & 65 & 40 kDa, respectively.

研究背景

GMP Glypican-3 (GPC3) 蛋白是一种细胞表面蛋白聚糖,在对发育过程至关重要的关键信号通路中发挥着复杂的调节作用。通过其 GPI 锚定,GPC3 通过与刺猬受体 PTC1 竞争与刺猬蛋白的结合,负向调节刺猬信号通路,导致复杂的内化和随后的溶酶体降解。同时,它通过与 Wnt 受体 Frizzled 结合,对经典和非经典 Wnt 信号通路发挥正向调节作用,增强 FrizzledWnt 配体之间的相互作用。GPC3 与 CD81 结合,减少游离 CD81 与转录抑制因子 HHEX 结合的可用性,导致 HHEX 核易位和转录抑制。此外,GPC3 抑制 DPP4 的二肽基肽酶活性。从功能上讲,GPC3 在肢体模式、骨骼发育、肾分支形态发生和冠状血管发育中发挥着关键作用。它还调节生长因子 BMP2、BMP7 和 FGF7 对肾分支形态发生的影响,并有助于调节原肠胚形成过程中的细胞运动。GPC3 以二硫键形成的异二聚体形式存在,并与多种分子相互作用,包括 DPP4、FGF2、WNT5A、WNT3A、WNT7B、刺猬蛋白 SHH 和 IHH、Wnt 受体 FZD4、FZD7 和 FZD8,显示其在协调发育过程中的关键作用。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P51654 (Q25-H559)

基因 ID
蛋白结构
N-term
GPC3 (Q25-H559)
Accession # P51654
hFc
C-term
同用名
rHuGlypican-3/GPC3, Fc; Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5
氨基酸序列

QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVH

分子量

100-120&65&40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Glypican-3/GPC3 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Glypican-3/GPC3 Protein, Human (HEK293, Fc)
目录号:
HY-P70367
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