Glypican-3/GPC3 蛋白, Rhesus Macaque (HEK293, His)

GPC3 is a member of the heparan sulfate proteoglycans (HSPGs), and attaches to the cell surface by a glycosylphosphatidylinositol anchor. GPC3 is overexpressed in hepatocellular carcinoma (HCC) and can be used as a tumor marker for HCC. Membrane GPC3 can interact with several growth factors to promote the binding of growth factors to their receptors. GPC3 can form a complex with Wnt and activates Wnt signaling, leading to the HCC growth. In addition, GPC3 is also involved in the pathogenesis of Simpson-Golabi-Behmel overgrowth syndrome, regulates cell growth, regulates embryonic and fetal development^[1][2][3]. Glypican-3/GPC3 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Glypican-3/GPC3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Glypican-3/GPC3 Protein, Rhesus Macaque (HEK293, His) is 535 a.a., with molecular weight of 38 & 65-120 kDa, respectively.

GPC3 是硫酸乙酰肝素蛋白聚糖 (HSPG) 的成员，通过糖基磷脂酰肌醇锚附着在细胞表面。GPC3 在肝细胞癌 (HCC) 中过度表达，可作为 HCC 的肿瘤标志物。GPC3 在肝脏中发挥作用致癌作用^[1]。具体而言，膜 GPC3 可以与多种生长因子 (例如：Wnt、Hedgehogs、骨形态发生因子和 FGF) 相互作用，促进生长因子与其受体结合。GPC3 可以与 Wnt 形成复合物并激活 Wnt 信号传导，导致 HCC 生长^[2]。
此外，GPC3 可以调节细胞生长。GPC3 在中胚层胚胎组织中高表达，GPC3 基因的缺失与 Simpson-Golabi-Behmel 过度生长综合征的发病机制有关，GPC3 与 IGF2 的相互作用可以减少 IGF2 介导的体内 (in vivo) 生长，表明 GPC3 负调控胚胎和胎儿发育。此外，GPC3 还是负转录调节因子和肿瘤抑制因子，可抑制乳腺癌、卵巢癌和肺癌细胞的生长^[2][3]。
GPC3 在 Arg358 和 Ser359 之间有一个切割位点，可以被 Furin 蛋白酶切割^[2]。

Rhesus Macaque

HEK293

C-6*His

XP_005594665.1 (Q25-H559)

102137748  [NCBI]

QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSVPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMMKVKNQLRFLAELAYDLDVDDVPGNNQQATPKDNEISTFHNLGNVH

38&65-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.