2. 重组蛋白
  3. CAR-T related Proteins
  4. GPC-3
  5. Glypican-5/GPC5 蛋白, Human (sf9, His)

Glypican-5/GPC5 蛋白, Human (sf9, His)

目录号: HY-P73079
产品使用指南

Glypican-5 (GPC5) 蛋白是一种细胞表面蛋白聚糖，其特征是与硫酸乙酰肝素特异性结合。作为一种蛋白聚糖，GPC5 由与硫酸乙酰肝素链共价连接的核心蛋白组成。Glypican-5/GPC5 蛋白, Human (sf9, His) 是重组的 Glypican-5/GPC5 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。Glypican-5/GPC5 蛋白, Human (sf9, His) 全长 530 个氨基酸，分子量约为 ~60.5 kDa。

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描述

Glypican-5 (GPC5) protein is a cell surface proteoglycan characterized by specific binding to heparan sulfate. As a proteoglycan, GPC5 consists of a core protein covalently linked to heparan sulfate chains. Glypican-5/GPC5 Protein, Human (sf9, His) is the recombinant human-derived Glypican-5/GPC5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Glypican-5/GPC5 Protein, Human (sf9, His) is 530 a.a., with molecular weight of ~60.5 kDa.
研究背景

Glypican-5 (GPC5) 蛋白是一种细胞表面蛋白聚糖，其特点是与硫酸乙酰肝素的特异性结合。作为一种蛋白聚糖，GPC5 由共价连接到硫酸乙酰肝素链的核心蛋白组成。这种蛋白质在细胞表面的存在表明它参与细胞信号传导和通讯。硫酸乙酰肝素链以其与各种生长因子和其他信号分子相互作用的能力而闻名，可能有助于 GPC5 在调节信号通路和细胞过程中发挥作用。GPC5 的独特特征（包括其与硫酸乙酰肝素的关联）表明其对细胞功能的重大贡献，并凸显了其作为进一步研究和治疗干预目标的潜力。
种属

Human
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

P78333 (E25-T554)
基因 ID
蛋白结构
N-term
GPC5 (E25-T554)
Accession # P78333
His
C-term
同用名
Glypican proteoglycan 5; GPC5
氨基酸序列

MDAQTWPVGFRCLLLLALVGSARSEGVQTCEEVRKLFQWRLLGAVRGLPDSPRAGPDLQVCISKKPTCCTRKMEERYQIAARQDMQQFLQTSSSTLKFLISRNAAAFQETLETLIKQAENYTSILFCSTYRNMALEAAASVQEFFTDVGLYLFGADVNPEEFVNRFFDSLFPLVYNHLINPGVTDSSLEYSECIRMARRDVSPFGNIPQRVMGQMGRSLLPSRTFLQALNLGIEVINTTDYLHFSKECSRALLKMQYCPHCQGLALTKPCMGYCLNVMRGCLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRNSEETLANRRKEFINSLRLYRSFYGGLADQLCANELAAADGLPCWNGEDIVKSYTQRVVGNGIKAQSGNPEVKVKGIDPVINQIIDKLKHVVQLLQGRSPKPDKWELLQLGSGGGMVEQVSGDCDDEDGCGGSGSGEVKRTLKITDWMPDDMNFSDVKQIHQTDTGSTLDTTGAGCAVAT

分子量

Approximately 60.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Glypican-5/GPC5 Protein, Human (sf9, His) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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