1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors Neurotrophic Factors Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. IL-6R gp130/CD130
  5. Glycoprotein 130 (gp130)
  6. gp130/IL6ST 蛋白, Human (HEK293, His-Fc)

gp130/IL6ST 蛋白, Human (HEK293, His-Fc)

目录号: HY-P75795
产品使用指南

Glycoprotein 130 (gp130) 是一种跨膜蛋白,是白细胞介素 6 (IL-6) 家族的常见信号转导受体亚基。gp130 抑制细胞生长和迁移,降低 IL-6 诱导的 STAT3 磷酸化,降低 IL-6 诱导的 Ki67 增加。gp130 具有抗肿瘤、抗炎和抗伤害活性。gp130/IL6ST 蛋白, Human (HEK293, His-Fc) 是一种重组蛋白,C 端带有 His-Fc 标签,由 618 个氨基酸 (M1-I618) 组成,在 HEK293 细胞中表达。

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描述

Glycoprotein 130 (gp130) is a trans-membrane protein, is the common signal-transducing receptor subunit of the interleukin-6 (IL-6) family[1]. gp130 inhibits the cell growth and migration[3], decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67. gp130 shows anti-tumor, anti-inflammation and anti-nociceptive activity[4][5]. gp130/IL6ST Protein, Human (HEK293, His-Fc) is a recombinant protein with a His-Fc label that consists of 618 amino acids (M1-I618) and is produced by HEK293 cells.

研究背景

Glycoprotein 130 mRNA is ubiquitously expressed in the human body,exhibiting highest levels in saphenous vein, pericardium, ovary, omental adipose tissue, peritoneum, spleen lymphnodes, and trigeminal ganglia. And gp130 expression is significantly increased in adult organs compared to fetal organs. Moreover, gp130 is highly expressed in multiple types of cancers compared to normal tissues[3].
The amino acid sequence of human gp130 protein has low homology between mouse and rat gp130 protein.
gp130 signaling is initiated when autocrine or paracrine cyto-kines induce the dimerization of gp130 and an α-receptor subunit to form the receptor complex, leading to185the activation of downstream signaling cascades, such as the JAK/STAT, the PI3K/Akt, and the Ras/Raf/Mek/Erk1/2MAPK pathways. The activation of JAK/Stat3 pathway induces its target gene expression, resulting in cell growth and proliferation (cyclin D, CDC25 A, c-Myc, survivin), survival (survivin, Bcl-2, Bcl-XL), angiogenesis (VEGF), and cell migration (MMP-2, MMP-7, and MMP-9)[3]. gp130 is a IL-6 antagonist shows anti-tumor, anti-inflammation and antinociceptive activity[4]. gp130 decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67[5].

体外研究
(In Vitro)

gp130 (human) (0-1.14 µg/mL; 24 h) inhibits the cell growth in a dose-dependent manner and induces apoptosis in HepG2 cells[5].
Glycoprotein 130 (human) (0-2 ng/mL; 48 h) significantly decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67 in HepG2 cells[5].

体内研究
(In Vivo)

Glycoprotein 130 (human) (5 µg/mL; i.p., 200 µL; every third day for 12 days) shows antinociceptive effect in antigen-induced arthritis rats[4].
Glycoprotein 130 (human) (2 µg/mL; i.a.; 50 µL; every third day for 12 days) shows antinociceptive effect in antigen-induced arthritis rats[4].
Glycoprotein 130 (human) (1.14 µg/kg; i.v.; every 3 days for 35 days) suppresses tumor growth in NOD/SCID mice with HepG2 cells injection[5].

生物活性

Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cell and the ED50 is typically 0.2-0.8 μg/mL in the presence of 50 ng/mL human IL-6sR and 100 ng/mL human IL-6.

种属

Human

表达系统

HEK293

标签

C-hFc;C-His

蛋白编号

P40189 (M1-I618)

基因 ID

3572  [NCBI]

中文名
重组人 gp130/IL6ST 蛋白
同用名
Interleukin-6 receptor subunit beta; IL-6RB; CDw130; gp130; CD130; IL6ST
分子量

125-140 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
gp130/IL6ST Protein, Human (HEK293, His-Fc)
目录号:
HY-P75795
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