1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins
  3. Interleukin & Receptors Neurotrophic Factors Epithelial cell CD Proteins Endothelial cell CD Proteins Cytokine Receptors
  4. IL-6R gp130/CD130
  5. Glycoprotein 130 (gp130)
  6. gp130/IL6ST 蛋白, Mouse (HEK293, His)

gp130/IL6ST 蛋白, Mouse (HEK293, His)

目录号: HY-P76371
COA 产品使用指南

Glycoprotein 130 (gp130) 是一种跨膜蛋白,是白细胞介素 6 (IL-6) 家族的常见信号转导受体亚基。gp130 抑制细胞生长和迁移,降低 IL-6 诱导的 STAT3 磷酸化,降低 IL-6 诱导的 Ki67 增加。gp130 具有抗肿瘤、抗炎和抗伤害活性。gp130/IL6ST 蛋白, Mouse (617a.a, HEK293, His) 是一种重组蛋白,C 端带有 His 标签,在 HEK293 细胞中表达。

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2 μg ¥350 In-stock
10 μg ¥970 In-stock
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50 μg ¥2950 In-stock
100 μg ¥4700 In-stock
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描述

Glycoprotein 130 (gp130) is a trans-membrane protein, is the common signal-transducing receptor subunit of the interleukin-6 (IL-6) family[1]. gp130 inhibits the cell growth and migration[3], decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67. gp130 shows anti-tumor, anti-inflammation and anti-nociceptive activity[4][5]. gp130/IL6ST Protein, Mouse (617a.a, HEK293, His) is a recombinant protein with a His label and is produced by HEK293 cells.

研究背景

糖蛋白130 mRNA在人体内广泛表达,在隐静脉、心包、卵巢、网膜脂肪组织、腹膜、脾淋巴结和三叉神经节中表达水平最高。与胎儿器官相比,成人器官中gp130的表达显着增加。此外,与正常组织相比,gp130 在多种类型的癌症中高表达[3]
人 gp130 蛋白的氨基酸序列与小鼠和大鼠 gp130 蛋白的同源性较低。
当自分泌或旁分泌细胞因子诱导 gp130 和 α 受体亚基二聚化形成受体复合物时,gp130 信号传导启动,导致下游信号级联的激活,例如 JAK/STAT、PI3K/Akt、和 Ras/Raf/Mek/Erk1/2MAPK 通路。JAK/Stat3通路的激活诱导其靶基因表达,导致细胞生长、增殖(cyclin D、CDC25 A、c-Myc、survivin)、生存(survivin、Bcl-2、Bcl-XL)、血管生成 (VEGF) 和细胞迁移(MMP-2、MMP-7 和 MMP-9)[3]
gp130 是一种 IL-6 拮抗剂,具有抗-肿瘤、抗炎和抗伤害活性[4]。gp130 降低 IL-6 诱导的 STAT3 磷酸化,并降低 IL-6 诱导的 Ki67[5] 增加。

生物活性

1.Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cells and the ED50 is typically 0.6-3 μg/mL in the presence of 50 ng/mL human IL-6sR and 100 ng/mL human IL-6.
2.Measured by its ability to bind mouse IL-11Ra in a functional ELISA.
3.Immobilized Human gp130 at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha that produces 50% of the optimal binding response is found to be approximately 7.854 ng/mL.
4.Measured by its ability to inhibit IL-6-dependent proliferation of TF-1 cells. The ED50 this effect is 0.1185 ng/mL in the presence of 4 μg/mL Recombinant Human IL-6 R alpha , corresponding to a specific activity is 8.44×10^6 units/mg.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q00560/NP_034690 (Q23-E617)

基因 ID

16195  [NCBI]

蛋白结构
N-term
gp130 (E19-E617)
Accession # Q00560
His
C-term
中文名
重组小鼠gp130/IL6ST 蛋白 (His)
同用名
Interleukin-6 receptor subunit beta; IL-6R-beta; IL-6RB; gp130; CD130
氨基酸序列

QLLEPCGYIYPEFPVVQRGSNFTAICVLKEACLQHYYVNASYIVWKTNHAAVPREQVTVINRTTSSVTFTDVVLPSVQLTCNILSFGQIEQNVYGVTMLSGFPPDKPTNLTCIVNEGKNMLCQWDPGRETYLETNYTLKSEWATEKFPDCQSKHGTSCMVSYMPTYYVNIEVWVEAENALGKVSSESINFDPVDKVKPTPPYNLSVTNSEELSSILKLSWVSSGLGGLLDLKSDIQYRTKDASTWIQVPLEDTMSPRTSFTVQDLKPFTEYVFRIRSIKDSGKGYWSDWSEEASGTTYEDRPSRPPSFWYKTNPSHGQEYRSVRLIWKALPLSEANGKILDYEVILTQSKSVSQTYTVTGTELTVNLTNDRYVASLAARNKVGKSAAAVLTIPSPHVTAAYSVVNLKAFPKDNLLWVEWTPPPKPVSKYILEWCVLSENAPCVEDWQQEDATVNRTHLRGRLLESKCYQITVTPVFATGPGGSESLKAYLKQAAPARGPTVRTKKVGKNEAVLAWDQIPVDDQNGFIRNYSISYRTSVGKEMVVHVDSSHTEYTLSSLSSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIE

分子量

80-90 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
gp130/IL6ST Protein, Mouse (HEK293, His)
目录号:
HY-P76371
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