GRIK2 蛋白, Human (HEK293, hFc)

GRIK2 is an ionotropic glutamate receptor that plays an important role in central nervous system synapses, responding to L-glutamate as an excitatory neurotransmitter. Upon binding of L-glutamate, GRIK2 undergoes a conformational change that opens cation channels that convert chemical signals into electrical impulses. GRIK2 Protein, Human (HEK293, hFc) is the recombinant human-derived GRIK2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GRIK2 Protein, Human (HEK293, hFc) is 530 a.a., with molecular weight of ~100-115 kDa.

GRIK2 是一种离子型谷氨酸受体，是中枢神经系统突触的关键成分，其中 L-谷氨酸充当兴奋性神经递质。与 L-谷氨酸结合后，受体会发生构象变化，促进阳离子通道的打开并将化学信号转化为电脉冲。随后，受体迅速脱敏并进入短暂的失活状态，其标志是存在结合的激动剂。除了其离子型谷氨酸受体活性外，GRIK2 还在调节 NETO2 的细胞表面表达方面发挥作用，并作为温度感受器发挥作用，赋予对低温的敏感性，特别是在背根神经节神经元中。

Measured by its ability to inhibit cell migration of AGS cells.

Human

HEK293

C-hFc

Q13002/NP_001159719.1 (T32-P561)

2898  [NCBI]

TTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKDAKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGKPANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGAQDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSP

Approximately 100-115 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.