2. 重组蛋白
  3. Enzymes & Regulators
  4. Serine/Threonine Kinase Proteins
  5. G protein-coupled receptor kinases (GRKs)
  6. G Protein-coupled Receptor Kinase 6 (GRK6)
  7. GRK6 蛋白, Human (sf9, His-GST)

GRK6 蛋白, Human (sf9, His-GST)

目录号: HY-P75799
产品使用指南

GRK6 选择性磷酸化激活的 G 蛋白偶联受体，触发 β-抑制蛋白介导的脱敏、内化和信号传导事件。它与纹状体中的 D2 样多巴胺受体和趋化因子受体 CXCR4 脱敏有关，而趋化因子受体 CXCR4 对于 CXCL12 诱导的细胞趋化性至关重要。此外，如体外观察到的，GRK6 在 Wnt 信号传导过程中磷酸化视紫红质 (RHO) 和非 GPCR LRP6。GRK6 蛋白, Human (sf9, His-GST) 是重组的 GRK6 蛋白，由 Sf9 insect cells 表达，带有 N-GST, N-His 标签。GRK6 蛋白, Human (sf9, His-GST) 全长 589 个氨基酸，分子量约为 ~85 kDa。

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描述

GRK6 selectively phosphorylates activated G protein-coupled receptors, triggering beta-arrestin-mediated desensitization, internalization, and signaling events. It is implicated in desensitizing D2-like dopamine receptors in the striatum and chemokine receptor CXCR4, crucial for CXCL12-induced cell chemotaxis. Additionally, GRK6 phosphorylates rhodopsin (RHO) and the non-GPCR LRP6 during Wnt signaling, as observed in vitro. GRK6 Protein, Human (sf9, His-GST) is the recombinant human-derived GRK6 protein, expressed by Sf9 insect cells , with N-GST, N-His labeled tag. The total length of GRK6 Protein, Human (sf9, His-GST) is 589 a.a., with molecular weight of ~85 kDa.
研究背景

GRK6 是一种选择性磷酸化 G 蛋白偶联受体激活形式的蛋白质，启动下游过程，例如 β-抑制蛋白介导的受体脱敏、内化和对其功能下调至关重要的信号事件。它参与纹状体中 D2 样多巴胺受体和趋化因子受体 CXCR4 的脱敏，在 CXCL12 诱导的细胞趋化性中发挥关键作用。此外，GRK6 已被证明可以在体外磷酸化视紫红质 (RHO) 和 Wnt 信号传导过程中的非 G 蛋白偶联受体 LRP6，表明其参与多种细胞途径。
种属

Human
表达系统

Sf9 insect cells
标签

N-GST;N-His
蛋白编号

P43250-2 (M1-R589)
基因 ID
蛋白结构
N-term
His-GST
GRK6 (M1-R589)
Accession # P43250-2
C-term
同用名
G protein-coupled receptor kinase 6; GRK6; GPRK6
分子量

Approximately 85 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

GRK6 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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