HCC-1/CCL14 蛋白, Human (66a.a, His)

HCC-1/CCL14 Protein, Human (66a.a, His) is a CC chemokine with weak activity against human monocytes, promotes monocyte, eosinophil and T-lymphocyte chemotaxis, and mediates allergic airway inflammation and cancer. HCC-1/CCL14 Protein, Human (66a.a, His) is a recombinant human HCC-1/CCL14 (G28-N93) expressed by E. coli with a his tag at the nitrogen end^[1][2].

CCL14，也称为HCC-1，是一种人血浆趋化因子，最初从慢性肾功能衰竭患者的血液滤液中收集和纯化。CCL14属于CC趋化因子的小细胞因子家族，是一簇位于人类17号染色体上的趋化因子，可在多种组织中表达，包括脾、骨髓、肝脏、肌肉和肠。CCL14对人单核细胞的活性较弱，对T淋巴细胞、中性粒细胞和嗜酸性粒细胞无活性。CCL14 对人单核细胞有弱活性，对 T 淋巴细胞、中性粒细胞和嗜酸性粒细胞无活性。CCL14 作为蛋白质前体，需要蛋白质水解以获得受体亲和力，经过加工可产生含有 74 个氨基酸的成熟活性蛋白质。加工后的 HCC-1(9-74) 是一种趋化因子，可吸引单核细胞、嗜酸性粒细胞和 T 细胞，并与 CCR1、CCR3 和 CCR5 趋化因子受体结合^[2]。与未修饰的趋化因子相比，CCL14 趋化因子的翻译后修饰（例如 N 末端截短和糖基化）也会导致差异信号传导。例如，全长 CCL14(1-74) 和截短亚型 CCL14(9-74) 均与非典型趋化因子受体 2 (ACKR2) 结合，但只有截短 CCL14(9-74) 显示出 β-restin 倾向并诱导与 CCL14(1-74) 相比，ACKR2 的受体内化。同时，CCL14(1-74) 是趋化因子受体 CCR1 的弱激动剂，但在蛋白水解为 CCL14(9-74) 后其活性显着增强^[1]。CCL14已被证明可以通过抑制肝细胞癌（HCC）细胞的细胞周期进程和促进细胞凋亡来抑制HCC细胞增殖。CCL14在体内抑制裸鼠体内HCC肿瘤的生长。CCL14还参与多种疾病的发病机制和进展，包括过敏性气道炎症和某些癌症^[2]。

CCL14 (1 nM, 22 h) can increase trophoblast adhesion to fibronectin by 3-fold and increase expression of extracellular matrix and adhesion molecules such as collagen COL5A1, matrix metalloproteinase MMP12 in human choriocarcinoma-primary trophoblast hybrid AC1M-88 cell line^[3].

Human

E. coli

N-His

Q16627 (G28-N93)

6358  [NCBI]

Approximately 12 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Siyao Wang, et al. Glycosylation Regulates N-Terminal Proteolysis and Activity of the Chemokine CCL14. ACS Chem Biol. 2021 Jun 18;16(6):973-981.  [Content Brief]

  [2]. Mengxuan Zhu, et al. CCL14 serves as a novel prognostic factor and tumor suppressor of HCC by modulating cell cycle and promoting apoptosis. Cell Death Dis. 2019 Oct 22;10(11):796.  [Content Brief]

  [3]. Natalie J Hannan, et al. CX3CL1 and CCL14 regulate extracellular matrix and adhesion molecules in the trophoblast: potential roles in human embryo implantation. Biol Reprod. 2008 Jul;79(1):58-65.  [Content Brief]