1. 重组蛋白
  2. Viral Proteins
  3. HCoV-HKU1 Spike/S1 蛋白 (Q5MQD0, HEK293, His)

HCoV-HKU1 Spike/S1 蛋白 (Q5MQD0, HEK293, His)

目录号: HY-P76388
产品使用指南

HCoV-HKU1 Spike/S1 蛋白通过将病毒颗粒附着在宿主细胞膜上,启动感染过程,在感染中发挥关键作用。作为 I 类病毒融合蛋白,它通过动态构象转变促进病毒粒子和宿主细胞之间的膜融合。具有七肽重复序列的卷曲螺旋区域在融合过程中形成发夹三聚体结构,使融合肽靠近C末端区域并驱动病毒和靶细胞膜的融合。HCoV-HKU1 Spike/S1 蛋白 (Q5MQD0, HEK293, His) 是重组的 HCoV-HKU1 刺突/S1 蛋白,由 HEK293 表达,带有 C-His 标签。HCoV-HKU1 Spike/S1 蛋白 (Q5MQD0, HEK293, His) 全长 760 个氨基酸,分子量为 130-140 KDa。

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描述

The HCoV-HKU1 Spike/S1 Protein plays a pivotal role in infection by attaching the virion to the host cell membrane, initiating the infection process. As a class I viral fusion protein, it facilitates membrane fusion between the virion and host cell through dynamic conformational transitions. The coiled coil regions, with heptad repeats, form a trimer-of-hairpins structure during fusion, bringing the fusion peptide close to the C-terminal region and driving the fusion of viral and target cell membranes. HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His) is the recombinant Virus-derived HCoV-HKU1 Spike/S1 protein, expressed by HEK293, with C-His labeled tag. The total length of HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His) is 760 a.a., with molecular weight of 130-140 KDa.

研究背景

HCoV-HKU1 Spike/S1 蛋白通过与宿主受体相互作用将病毒体附着到宿主细胞膜上,从而启动感染,从而在感染过程中发挥关键作用。作为 I 类病毒融合蛋白,它促进病毒粒子和细胞膜的融合。该蛋白质经历动态构象转变,表现出至少三种状态:融合前天然状态、发夹前中间状态和融合后发夹状态。在融合过程中,以七肽重复为特征的卷曲线圈区域形成发夹三聚体结构。这种排列将融合肽定位在紧邻胞外域的C端区域的位置,这种结构被认为能够驱动病毒和靶细胞膜的并置和随后的融合。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

Q5MQD0 (M1-R760)

基因 ID

3200426  [NCBI]

蛋白结构
N-term
HCoV-HKU1 S1 (M1-R760)
Accession # Q5MQD0
His
C-term
同用名
Human coronAvirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His)
分子量

130-140 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His)
目录号:
HY-P76388
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