1. 重组蛋白
  2. Cytokines and Growth Factors
  3. HDGF 蛋白, Human (His)

HDGF 蛋白, Human (His)

目录号: HY-P70368
产品使用指南

HDGF 蛋白作为转录抑制蛋白,之前被认为对成纤维细胞具有促有丝分裂活性,并且是一种肝素结合蛋白。然而,最近的研究表明,它对成纤维细胞不具有促有丝分裂活性,并且不与肝素结合。HDGF 蛋白, Human (His) 是重组的 HDGF 蛋白,由 E. coli 表达,带有 C-6*His 标签。HDGF 蛋白, Human (His) 全长 100 个氨基酸,分子量约为 ~14.0 kDa。

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50 μg   询价  
100 μg   询价  

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描述

The HDGF protein acts as a transcriptional repressor and was previously thought to have mitogenic activity for fibroblasts and be a heparin-binding protein. However, recent studies have shown that it does not exhibit mitogenic activity for fibroblasts and does not bind heparin. HDGF Protein, Human (His) is the recombinant human-derived HDGF protein, expressed by E. coli , with C-6*His labeled tag. The total length of HDGF Protein, Human (His) is 100 a.a., with molecular weight of ~14.0 kDa.

研究背景

肝细胞瘤衍生生长因子 (HDGF) 具有转录抑制因子和成纤维细胞有丝分裂因子的双重作用。虽然它作为转录调节的阻遏物,影响细胞过程,但它也表现出促有丝分裂活性,特别是对于成纤维细胞。HDGF 已被鉴定为肝素结合蛋白,这表明它参与与肝素的相互作用并可能调节细胞反应。然而,值得注意的是,存在相互矛盾的报道,一些研究表明 HDGF 缺乏成纤维细胞有丝分裂活性,并且不与肝素结合。这些发现强调了 HDGF 功能的复杂性,展示了其在转录控制、有丝分裂信号传导以及与肝素的潜在相互作用中的不同作用。

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

P51858 (M1-Y100)

基因 ID
蛋白结构
N-term
HDGF (M1-Y100)
Accession # P51858
6*His
C-term
同用名
rHuHepatoma-derived growth factor/HDGF, His; Hepatoma-Derived Growth Factor; HDGF; High Mobility Group Protein 1-Kike 2; HMG-1L2; HDGF; HMG1L2
氨基酸序列

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGY

分子量

Approximately 14.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HDGF Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HDGF Protein, Human (His)
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