Heparanase/HPSE 蛋白, Human (CHO, His)

Heparanase (HPSE) functions as an endoglycosidase, playing a crucial role in the cleavage of heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. It is implicated in various cellular processes, including facilitating cell migration associated with metastasis, wound healing, and inflammation. Heparanase also plays a role in cell adhesion to the ECM, independent of its enzymatic activity, and induces AKT1/PKB phosphorylation, thereby increasing cell mobility and invasion. Additionally, Heparanase is involved in the regulation of osteogenesis, promotes angiogenesis through up-regulation of SRC-mediated activation of VEGF, and is implicated in hair follicle inner root sheath differentiation and hair homeostasis. Heparanase/HPSE Protein, Human (CHO, His) is the recombinant human-derived Heparanase/HPSE protein, expressed by CHO , with N-6*His labeled tag.

Heparanase (HPSE) 作为糖苷内切酶，在硫酸乙酰肝素蛋白聚糖 (HSPG) 裂解为硫酸乙酰肝素侧链和核心蛋白聚糖的过程中发挥着至关重要的作用。这种酶活性有助于细胞外基质 (ECM) 降解和重塑，选择性地裂解具有特定硫酸化模式的葡萄糖醛酸单元和 N-磺基葡萄糖胺单元内的连接。虽然 HPSE 在中性 pH 值下主要不活跃，但在酸性条件下 (例如在肿瘤侵袭和炎症过程中) 会变得活跃。这种蛋白质参与多种细胞过程，包括促进与转移、伤口愈合和炎症相关的细胞迁移。它可以增强多配体的脱落，促进骨髓瘤中的内皮侵袭和血管生成，并通过在组织因子和激活因子 VII 存在的情况下增加激活因子 X 的生成来充当促凝血剂。值得注意的是，HPSE 还在细胞与 ECM 的粘附中发挥作用 (与其酶活性无关)，并诱导 AKT1/PKB 磷酸化，从而增加细胞迁移性和侵袭性。此外，HPSE 参与成骨调节，通过上调 SRC 介导的 VEGF 激活促进血管生成，并参与毛囊内根鞘分化和毛发稳态^{[1][2][3][4][5]}。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its binding ability in a functional ELISA. Immobilized HPSE at 2 μg/ml (100μl/well) can bind HPSE antibody. The ED₅₀ for this effect is 0.2357 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized HPSE at 2 μg/ml (100μl/well) can bind HPSE antibody. The ED₅₀ for this effect is 0.2357 μg/mL.

Human

CHO

N-6*His

AAD41342.1 (Q36-I543)

10855  [NCBI]

Full length of Mature Protein

Approximately 78 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.