1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. EGF Superfamily Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. EGFR/ErbB family
  5. ErbB4/HER4
  6. HER4 蛋白, Human (HEK293, His)

HER4 蛋白, Human (HEK293, His)

目录号: HY-P70841
COA 产品使用指南

HER4 是神经调节蛋白和 EGF 家族成员的重要酪氨酸蛋白激酶受体,可指导心脏、中枢神经系统和乳腺发育。它对于心肌分化、出生后心肌细胞增殖、神经嵴细胞迁移、轴突引导和乳腺功能至关重要。HER4 蛋白, Human (HEK293, His) 是重组的 HER4 蛋白,由 HEK293 表达,带有 C-6*His 标签。HER4 蛋白, Human (HEK293, His) 全长 624 个氨基酸,分子量为 94-107 kDa。

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规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥2800 In-stock
100 μg   询价  

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描述

HER4 is an important tyrosine protein kinase receptor for members of the neuregulin and EGF families that directs heart, central nervous system, and mammary gland development. It is critical for myocardial differentiation, postnatal cardiomyocyte proliferation, neural crest cell migration, axon guidance, and mammary gland function. HER4 Protein, Human (HEK293, His) is the recombinant human-derived HER4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of HER4 Protein, Human (HEK293, His) is 624 a.a., with molecular weight of 94-107 kDa.

研究背景

HER4是一种酪氨酸蛋白激酶,是神经调节蛋白和EGF家族成员的关键细胞表面受体,在心脏、中枢神经系统和乳腺的发育以及基因转录、细胞增殖、分化、迁移和凋亡。它对于胚胎发育和出生后心肌细胞增殖过程中正常的心肌分化至关重要。此外,HER4是胚胎中枢神经系统正常发育所必需的,特别是神经嵴细胞迁移和轴突引导,以及乳腺分化和泌乳诱导。作为神经调节蛋白 NRG1、NRG2、NRG3、NRG4 和 EGF 家族成员 BTC、EREG 和 HBEGF 的受体,配体结合触发受体二聚化和自磷酸化,产生细胞内磷酸酪氨酸的多种组合,从而引发配体特异性和环境特异性细胞反应。HER4 介导 SHC1 磷酸化并激活 MAP 激酶 MAPK1/ERK2 和 MAPK3/ERK1。同工型 JM-A CYT-1 和 JM-B CYT-1 磷酸化 PIK3R1,激活磷脂酰肌醇 3-激酶和 AKT1,以防止细胞凋亡并促进细胞响应 NRG1 迁移。同种型 JM-A CYT-2 和 JM-B CYT-2 缺乏 PIK3R1 相互作用所需的磷酸酪氨酸,因此放弃了这些作用。同工型 JM-A CYT-1 和 JM-A CYT-2 的蛋白水解加工产生可溶性细胞内结构域 (4ICD),这些结构域易位至细胞核,促进 STAT5A 的核输入、乳腺上皮分化、细胞增殖和基因表达激活。此外,ERBB4 可溶性胞内结构域可以易位至线粒体,诱导细胞凋亡。

生物活性

Immobilized Human HER4, at 2 μg/mL (100μL/well) can bind Biotinylated Human NRG1 β1, the ED50 for this effect is 16.09 ng/mL.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q15303 (Q26-R649)

基因 ID
蛋白结构
N-term
HER4 (Q26-R649)
Accession # Q15303
6*His
C-term
同用名
Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4; ERBB4; HER4
氨基酸序列

QSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLSFLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNLTLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHGDPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNLVTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTINQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRRFSRGRICIESCNLYDGEFREFENGSICVECDPQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADPDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHAR

分子量

94-107 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HER4 Protein, Human (HEK293, His)
目录号:
HY-P70841
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