1. 重组蛋白
  2. Cytokines and Growth Factors
  3. HGF & Receptors
  4. HGF
  5. HGF 蛋白, Mouse (HEK293)

HGF 蛋白, Mouse (HEK293)

目录号: HY-P73928
COA 产品使用指南

HGF 蛋白是一种有效的有丝分裂原,可刺激成熟肝细胞生长并充当各种组织的肝营养因子。作为 MET 受体酪氨酸激酶的激活配体,HGF 结合并促进二聚化,通过 TMPRSS13 裂解激活 MAPK 信号传导。在结构上,HGF 包含通过二硫键连接的 α 链和 β 链。与 SRPX2 的相互作用增强其促有丝分裂活性。HGF 蛋白, Mouse (HEK293) 是重组的 HGF 蛋白,由 HEK293 表达,不带标签。HGF 蛋白, Mouse (HEK293) 全长 696 个氨基酸,分子量约为 ~93 kDa。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
20 μg ¥2350 In-stock
100 μg ¥6500 In-stock
> 100 μg   询价  

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MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and acts as a hepatotrophic factor for various tissues. As an activating ligand for MET receptor tyrosine kinase, HGF binds and promotes dimerization, activating MAPK signaling through TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains connected by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. HGF Protein, Mouse (HEK293) is the recombinant mouse-derived HGF protein, expressed by HEK293 , with tag free. The total length of HGF Protein, Mouse (HEK293) is 696 a.a., with molecular weight of ~93 kDa.

研究背景

HGF 蛋白是一种有效的有丝分裂原,可刺激成熟肝实质细胞的生长,并作为多种组织和细胞类型的肝营养因子。它充当受体酪氨酸激酶 MET 的激活配体,与其结合并促进其二聚化。HGF 还通过切割和激活 TMPRSS13 来激活 MAPK 信号传导。在结构上,HGF由通过二硫键连接的α链和β链组成。此外,它与 SRPX2 相互作用,促进 HGF 的促有丝分裂活性增加。

生物活性

1.Immobilized mouse HGF at 10 μg/mL (100 μL/well) can bind mouse MET-Fc and the EC50 is 0.05-0.12 μg/mL.
2.Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells and the ED50 is typically <4 ng/mL.

种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

Q08048 (Q33-L728)

基因 ID

15234  [NCBI]

蛋白结构
N-term
HGF (Q33-L728)
Accession # Q08048
C-term
同用名
Hepatocyte growth factor; F-TCF; Hepatopoeitin-A; HGF; HGFB; HPTA; SF
氨基酸序列

QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKKVNSADECANRCIRNRGFTFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKTCAHSAVNETDVPMETTECIQGQGEGYRGTSNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

分子量

Approximately 93 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

HGF Protein, Mouse (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HGF Protein, Mouse (HEK293)
目录号:
HY-P73928
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