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  4. Histone chaperone ASF1A/ASF1A 蛋白, Human (T7-His)

Histone chaperone ASF1A/ASF1A 蛋白, Human (T7-His)

目录号: HY-P70119
产品使用指南

组蛋白伴侣 ASF1A/ASF1A 蛋白是染色质动力学的关键参与者，支持组蛋白沉积、协调交换并在核小体组装和拆卸过程中帮助去除，多项研究已证实这一点。ASF1A 与 CAF-1 合作促进复制依赖性染色质组装，并与 HIRA 合作促进复制无关的组装。Histone chaperone ASF1A/ASF1A 蛋白, Human (T7-His) 是重组的 组蛋白伴侣 ASF1A 蛋白，由 E. coli 表达，带有 N-T7, C-6*His 标签。Histone chaperone ASF1A/ASF1A 蛋白, Human (T7-His) 全长 204 个氨基酸，分子量约为 ~22.0 kDa。

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描述

The histone chaperone ASF1A/ASF1A protein is a key player in chromatin dynamics, supporting histone deposition, coordinating exchange, and aiding in removal during nucleosome assembly and disassembly, as has been confirmed by multiple studies. ASF1A cooperates with CAF-1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent assembly. Histone chaperone ASF1A/ASF1A Protein, Human (T7-His) is the recombinant human-derived Histone chaperone ASF1A/ASF1A protein, expressed by E. coli , with N-T7, C-6*His labeled tag. The total length of Histone chaperone ASF1A/ASF1A Protein, Human (T7-His) is 204 a.a., with molecular weight of ~22.0 kDa.
研究背景

组蛋白伴侣 ASF1A/ASF1A 蛋白是染色质动力学的关键参与者，可促进组蛋白沉积，并在核小体组装和拆卸过程中协调组蛋白交换和去除。ASF1A 与染色质组装因子 1 (CAF-1) 协同作用，促进复制依赖性染色质组装，并与 HIRA 结合，促进复制无关的染色质组装。值得注意的是，ASF1A 在同源重组介导的双链断裂修复中发挥作用，通过介导断裂处的组蛋白替换并促进 MMS22L-TONSL 复合物的募集和随后的 RAD51 加载来发挥作用。此外，ASF1A 与 importin-4 (IPO4) 一起参与组蛋白 H3-H4 二聚体的核输入，特异性识别并结合胞质溶胶中用 H3K9me1K14ac 和 H4K5K12ac 标记的新合成的组蛋白。ASF1A 对于衰老相关异染色质灶 (SAHF) 的形成和有效的衰老相关细胞周期退出至关重要，它与组蛋白 H3 和 H4 以及各种伙伴蛋白（如 CHAF1A、CHAF1B、RBBP4、CABIN1、HAT1、HIRA）相互作用、NASP、TAF1、UBN1、CDAN1、IPO4 和 CREBBP。
种属

Human
表达系统

E. coli
标签

N-T7;C-6*His
蛋白编号

Q9Y294 (M1-M204)
基因 ID
蛋白结构
N-term
T7
ASF1A (M1-M204)
Accession # Q9Y294
6*His
C-term
同用名
rHuHistone chaperone ASF1A/ASF1A, T7-His; Histone Chaperone ASF1A; Anti-Silencing Function Protein 1 Homolog A; hAsf1; hAsf1a; CCG1-Interacting Factor A; CIA; hCIA; ASF1A
氨基酸序列

MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

分子量

Approximately 22.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Histone chaperone ASF1A/ASF1A Protein, Human (T7-His) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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