1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. HS3ST1 蛋白, Human (sf9, His)

HS3ST1 蛋白, Human (sf9, His)

目录号: HY-P76974
产品使用指南

HS3ST1 蛋白利用 PAPS 关键催化硫酸盐转移至硫酸乙酰肝素 (HS) 中葡萄糖胺残基的第 3 位,代表 HS 生物合成中的限速步骤。这种活性对于抗凝剂硫酸乙酰肝素的形成和抗凝血酶五糖结合位点的完成至关重要。HS3ST1 蛋白, Human (sf9, His) 是重组的 HS3ST1 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。HS3ST1 蛋白, Human (sf9, His) 全长 287 个氨基酸,分子量约为 ~36 KDa。

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描述

The HS3ST1 protein utilizes PAPS to crucially catalyze the transfer of sulfate to position 3 of the glucosamine residue in heparan sulfate (HS), representing the rate-limiting step in HS biosynthesis. This activity is essential for the formation of the anticoagulant heparan sulfate and completion of the antithrombin pentasaccharide binding site. HS3ST1 Protein, Human (sf9, His) is the recombinant human-derived HS3ST1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of HS3ST1 Protein, Human (sf9, His) is 287 a.a., with molecular weight of ~36 KDa.

研究背景

HS3ST1 蛋白是一种利用 3'-磷酸-5'-腺苷酸硫酸盐 (PAPS) 作为底物的磺基转移酶,通过催化磺基转移到葡萄糖胺残基的 3 位,在硫酸乙酰肝素 (HS) 的生物合成中发挥关键作用在乙酰肝素中。这种酶活性代表 HS 生物合成中的限速步骤,对于抗凝剂硫酸乙酰肝素的形成至关重要。HS3ST1 促进的葡萄糖胺残基 3 位的硫酸化特别重要,因为它完成了抗凝血酶五糖结合位点的结构。通过调节硫酸乙酰肝素生物合成,HS3ST1 有助于产生参与抗凝的结构特异性分子,强调其在调节血液凝固过程中的关键作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

O14792 (R21-H307)

基因 ID
蛋白结构
N-term
His
HS3ST1 (R21-H307)
Accession # O14792
C-term
同用名
Heparan sulfate glucosamine 3-O-sulfotransferase 1; 3-OST-1; 3OST; 3OST1
分子量

Approximately 36 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HS3ST1 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HS3ST1 Protein, Human (sf9, His)
目录号:
HY-P76974
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