HS3ST1 蛋白, Human (sf9, His)

The HS3ST1 protein utilizes PAPS to crucially catalyze the transfer of sulfate to position 3 of the glucosamine residue in heparan sulfate (HS), representing the rate-limiting step in HS biosynthesis. This activity is essential for the formation of the anticoagulant heparan sulfate and completion of the antithrombin pentasaccharide binding site. HS3ST1 Protein, Human (sf9, His) is the recombinant human-derived HS3ST1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

HS3ST1 蛋白是一种利用 3'-磷酸-5'-腺苷酸硫酸盐 (PAPS) 作为底物的磺基转移酶，通过催化磺基转移到葡萄糖胺残基的 3 位，在硫酸乙酰肝素 (HS) 的生物合成中发挥关键作用在乙酰肝素中。这种酶活性代表 HS 生物合成中的限速步骤，对于抗凝剂硫酸乙酰肝素的形成至关重要。HS3ST1 促进的葡萄糖胺残基 3 位的硫酸化特别重要，因为它完成了抗凝血酶五糖结合位点的结构。通过调节硫酸乙酰肝素生物合成，HS3ST1 有助于产生参与抗凝的结构特异性分子，强调其在调节血液凝固过程中的关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

O14792 (R21-H307)

9957  [NCBI]

Full Length of Mature Protein

Approximately 36 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.