IFN-alpha 10/IFNA10 蛋白, Human (HEK293, Fc)

IFN-alpha 10 (IFNA10), belongs to type I interferon family, is produced by macrophages with antiviral activities^[1]. IFN-alpha 10/IFNA10 Protein, Human (HEK293, Fc) contains 166 a.a. (C24-D189), produced in HEK293 cells with a N-terminal hFc-tag.

IFN-α 10（IFNA10；IFN-α10）属于 α/β 干扰素 (IFN) 家族，由巨噬细胞产生，具有抗病毒活性^[1]。干扰素（IFN）最初被认为是一种在体外“干扰”病毒复制的物质。IFN-α/β及相关分子被归类为I型干扰素，其他两类分别为II型干扰素（IFN-γ）和III型干扰素（IFN-λ）^[2]。
干扰素刺激两种酶的产生：蛋白激酶和寡腺苷酸合成酶。干扰素α（IFNa）在多种癌症，尤其是毛细胞白血病、慢性粒细胞白血病等血液系统恶性肿瘤中表现出显着的生物活性^[3]。
IFNα-10参与JAK/STAT信号通路具有强信号传导效应 (EC₅₀=0.3 nM)，被认为是有效的调节剂，可降低 CTLA4 和 FOXP3 而不影响细胞活力。因此，调节性T细胞（Treg）作为调节外周自身反应性T淋巴细胞的关键细胞，IFNα-10调节Treg功能状态并破坏Treg^[4]。

IFN-alpha 1 (1^-11-1^-6 M; 24-26 h) results signifcant reduction of both CTLA4 and FOXP3 gene expression in regulatory T cells (Tregs) without affecting cell viability^[4].

Human

HEK293

N-hFc

P01566 (C24-D189)

3446  [NCBI]

Approximately 50 kDa.

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Kumagai Y, et al. Alveolar macrophages are the primary interferon-alpha producer in pulmonary infection with RNA viruses. Immunity. 2007 Aug;27(2):240-52.  [Content Brief]

  [2]. Zhang SY, et al. Inborn errors of interferon (IFN)-mediated immunity in humans: insights into the respective roles of IFN-alpha/beta, IFN-gamma, and IFN-lambda in host defense. Immunol Rev. 2008 Dec;226:29-40.  [Content Brief]

  [3]. Raj NB, et al. Identification of a novel virus-responsive sequence in the promoter of murine interferon-alpha genes. J Biol Chem. 1991 Jun 15;266(17):11360-5.  [Content Brief]

  [4]. Ding M, et al. Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells. Sci Rep. 2021 Aug 18;11(1):16767.  [Content Brief]