1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-alpha
  5. IFN-alpha 13
  6. IFN-alpha 13/IFNA1 蛋白, Human

IFN-alpha 13/IFNA1 蛋白, Human

目录号: HY-P72796
COA 产品使用指南

IFN-alpha 13 (IFNA13) 属于 I 型干扰素家族,由巨噬细胞产生而具有抗病毒活性。重组人 IFN-alpha 13/IFNA1 蛋白含有 166 个氨基酸 (C24-D189),由 E. coli 细胞产生,不带标签。

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规格 价格 是否有货 数量
10 μg ¥1250 In-stock
50 μg ¥3250 In-stock
100 μg   询价  
250 μg   询价  

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描述

IFN-alpha 13 (IFNA13), belongs to type I interferon family, is produced by macrophages with antiviral activities[1]. IFN-alpha 13/IFNA1 Protein, Human contains 166 a.a. (C24-D189), produced in E. coli cells with tag free.

研究背景

IFN-alpha 13 (IFNA13; IFN-α13) is produced by the macrophages, belongs to the alpha/beta interferon (IFN) family, a family of cytokines induced by viral infection and are primarily involved in antiviral defense of the cells[1]. Interferon (IFN) is originally identified as a substance ‘interfering’ with viral replication in vitro. IFN-α/β and related molecules are classified as type I IFNs, as for the other two types of type II IFN (IFN-γ) and type III IFNs (IFN-λ), respectively[2].
Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Interferon alpha (IFNa) shows significant biological activity in various cancers, paticularly haematological malignancies such as hairy cell leukaemia and chronic myelogenous leukaemia[3].
IFN-alpha13 exhibits acid-stable antiviral activity against Theiler's virus, Mengo virus, and vesicular stomatitis virus. Firstly, it is transcribed constitutively, independent of viral infection and of interferon regulatory factor-7 induction. Secondly, it contains two N-glycosylation sites, in contrast to other murine IFN-alpha subtypes that contain either one or no N-glycosylation site[4]. As for a wildly use of IFN in animal model, the sequence of amino acids in IFNA13 protein of human is very different from mouse (64.55%)

生物活性

The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P01562-1 (C24-E189)

中文名
重组人 IFN-alpha 13/IFNA1 蛋白
同用名
Interferon alpha-1/13; IFN-alpha-1/13; LeIF D; IFNA1; IFNA13
氨基酸序列

CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE

分子量

Approximately 19.5 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, containing 4% mannitol and 1% HSA.

内毒素含量

<1 EU/μg; determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IFN-alpha 13/IFNA1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-alpha 13/IFNA1 Protein, Human
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