IFN-gamma 蛋白, Human (HEK293)

IFN-gamma is a cytokine. IFN-gamma can exert anticancer effects by activating p53 and p21, leading to cell cycle arrest, and/or by activating IRF-1 and Caspase-1, leading to pyroptosis, and by activating IRF-1, Caspase-8, cytochrome c release from mitochondria, Caspase cascades, and inhibiting PARP, leading to intrinsic apoptotic signaling. IFN-gamma can induce fusion of human monocytes to form multinucleated giant cells and activate monocytes, increasing their ability to produce hydrogen peroxide, acid phosphatase, and plasminogen activator. IFN-gamma can enhance the sensitivity of human macrophages to lysis mediated by extracellular ATP. IFN-gamma upregulates the transcription and expression of TNF-α by inhibiting the expression of IL-10 and relieving the feedback inhibition of IL-10 on TNF-α. IFN-gamma Protein, Human (HEK293) is a recombinant interferon-gamma protein expressed in HEK293 cells without a tag.

IFN-gamma 属于干扰素家族。IFN-gamma 是一种细胞因子^[1]。IFN-gamma 可通过激活 p53 和 p21 导致细胞周期停滞，和/或通过激活 IRF-1 和 Caspase-1 引发焦亡，以及通过激活 IRF-1、Caspase-8、细胞色素 c 从线粒体释放、Caspase 级联反应和抑制 PARP 引发内在凋亡信号传导来发挥抗癌功效^[1]。IFN-gamma 能诱导人单核细胞融合形成多核巨细胞，并激活单核细胞，使其产生过氧化氢、酸性磷酸酶和纤溶酶原激活物的能力增强^[2]。IFN-gamma 可增强人巨噬细胞对细胞外 ATP 介导的裂解的敏感性^[3]。IFN-gamma 通过抑制 IL-10 的表达，解除 IL-10 对 TNF-α 的反馈抑制，从而上调 TNF-α 的转录和表达^[4]。IFN-gamma 可在 CD4⁺ T 辅助细胞 1 型淋巴细胞、CD8⁺ 细胞毒性淋巴细胞和自然杀伤 (NK) 细胞合成^[1]。IFN-gamma, Human 的活性形式是一种可溶性同源二聚体，每个二聚体表面的天冬酰胺 (N²⁵ 和 N⁹⁷) 上有两个 N 糖基化位点，N²⁵ 处的聚糖被岩藻糖基化，主要是具有唾液酸、半乳糖、甘露糖、N- 乙酰氨基葡萄糖和岩藻糖糖组成的复杂型唾液酸化寡糖，而 N⁹⁷ 处的聚糖是非岩藻糖基化的杂合高甘露糖结构，具有唾液酸、半乳糖、甘露糖和 N- 乙酰氨基葡萄糖的糖组成^[1]。

IFN-γ, Human (HEK293) (100 ng/mL; 72 h) 在卵巢癌细胞系 PEO1 中引起细胞死亡和 G₂ 期细胞周期阻滞^[1]。
IFN-γ, Human (E. coli) (0.1-1 nM) 在人单核细胞培养中诱导多核巨细胞形成，并增强 H₂O₂ 生成和溶酶体酶活性^[2]。
IFN-γ, Human (1-100 ng/mL; 4-18 h) 在 LPS 刺激的人单核细胞中抑制 IL-10 mRNA 和蛋白表达，剂量依赖性上调 TNF-α 转录及蛋白水平^[4]。

Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells.The ED₅₀ for this effect is ≤0.4622 ng/mL, corresponding to a specific activity is ≥2.164×10⁶ Unit/mg.

• 
  Measured by its ability to inhibit the proliferation of HT-29 cells. The ED₅₀ for this effect is 0.1895 ng/mL, corresponding to a specific activity is 5.277×10⁶ Unit/mg.

Human

HEK293

Tag Free

P01579 (Q24-Q166)

3458  [NCBI]

Full Length of Mature Protein

QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ

20-25 & 16-17kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 4% Mannitol, 2% Sucrose, 0.02% Tween80, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Razaghi A, et al. Improved therapeutic efficacy of mammalian expressed-recombinant interferon gamma against ovarian cancer cells. Exp Cell Res. 2017 Oct 1;359(1):20-29.  [Content Brief]

  [2]. Weinberg JB, et al. Recombinant human gamma-interferon induces human monocyte polykaryon formation. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4554-7.  [Content Brief]

  [3]. Blanchard DK, et al. IFN-gamma enhances sensitivity of human macrophages to extracellular ATP-mediated lysis. J Immunol. 1991 Oct 15;147(8):2579-85.  [Content Brief]

  [4]. Donnelly RP, et al. Inhibition of IL-10 expression by IFN-gamma up-regulates transcription of TNF-alpha in human monocytes. J Immunol. 1995 Aug 1;155(3):1420-7.  [Content Brief]