1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-γ
  5. IFN-gamma 蛋白, Human (HEK293)

IFN-gamma 蛋白, Human (HEK293)

目录号: HY-P70610
COA 产品使用指南 技术支持

IFN-gamma 是一种细胞因子。IFN-gamma 可通过激活 p53 和 p21 导致细胞周期停滞,和/或通过激活 IRF-1 和 Caspase-1 引发焦亡,以及通过激活 IRF-1、Caspase-8、细胞色素 c 从线粒体释放、Caspase 级联反应和抑制 PARP 引发内在凋亡信号传导来发挥抗癌功效。IFN-gamma 能诱导人单核细胞融合形成多核巨细胞,并激活单核细胞,使其产生过氧化氢、酸性磷酸酶和纤溶酶原激活物的能力增强。IFN-gamma 可增强人巨噬细胞对细胞外 ATP 介导的裂解的敏感性。IFN-gamma 通过抑制 IL-10 的表达,解除 IL-10 对 TNF-α 的反馈抑制,从而上调 TNF-α 的转录和表达。IFN-gamma 蛋白, Human (HEK293) 是重组的 干扰素-γ 蛋白,由 HEK293 表达,不带标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IFN-gamma is a cytokine. IFN-gamma can exert anticancer effects by activating p53 and p21, leading to cell cycle arrest, and/or by activating IRF-1 and Caspase-1, leading to pyroptosis, and by activating IRF-1, Caspase-8, cytochrome c release from mitochondria, Caspase cascades, and inhibiting PARP, leading to intrinsic apoptotic signaling. IFN-gamma can induce fusion of human monocytes to form multinucleated giant cells and activate monocytes, increasing their ability to produce hydrogen peroxide, acid phosphatase, and plasminogen activator. IFN-gamma can enhance the sensitivity of human macrophages to lysis mediated by extracellular ATP. IFN-gamma upregulates the transcription and expression of TNF-α by inhibiting the expression of IL-10 and relieving the feedback inhibition of IL-10 on TNF-α. IFN-gamma Protein, Human (HEK293) is a recombinant interferon-gamma protein expressed in HEK293 cells without a tag.

研究背景

IFN-gamma 属于干扰素家族。IFN-gamma 是一种细胞因子[1]。IFN-gamma 可通过激活 p53 和 p21 导致细胞周期停滞,和/或通过激活 IRF-1 和 Caspase-1 引发焦亡,以及通过激活 IRF-1、Caspase-8、细胞色素 c 从线粒体释放、Caspase 级联反应和抑制 PARP 引发内在凋亡信号传导来发挥抗癌功效[1]。IFN-gamma 能诱导人单核细胞融合形成多核巨细胞,并激活单核细胞,使其产生过氧化氢、酸性磷酸酶和纤溶酶原激活物的能力增强[2]。IFN-gamma 可增强人巨噬细胞对细胞外 ATP 介导的裂解的敏感性[3]。IFN-gamma 通过抑制 IL-10 的表达,解除 IL-10 对 TNF-α 的反馈抑制,从而上调 TNF-α 的转录和表达[4]。IFN-gamma 可在 CD4+ T 辅助细胞 1 型淋巴细胞、CD8+ 细胞毒性淋巴细胞和自然杀伤 (NK) 细胞合成[1]。IFN-gamma, Human 的活性形式是一种可溶性同源二聚体,每个二聚体表面的天冬酰胺 (N25 和 N97) 上有两个 N 糖基化位点,N25 处的聚糖被岩藻糖基化,主要是具有唾液酸、半乳糖、甘露糖、N- 乙酰氨基葡萄糖和岩藻糖糖组成的复杂型唾液酸化寡糖,而 N97 处的聚糖是非岩藻糖基化的杂合高甘露糖结构,具有唾液酸、半乳糖、甘露糖和 N- 乙酰氨基葡萄糖的糖组成[1]

体外研究
(In Vitro)

IFN-γ, Human (HEK293) (100 ng/mL; 72 h) 在卵巢癌细胞系 PEO1 中引起细胞死亡和 G2 期细胞周期阻滞[1]
IFN-γ, Human (E. coli) (0.1-1 nM) 在人单核细胞培养中诱导多核巨细胞形成,并增强 H2O2 生成和溶酶体酶活性[2]
IFN-γ, Human (1-100 ng/mL; 4-18 h) 在 LPS 刺激的人单核细胞中抑制 IL-10 mRNA 和蛋白表达,剂量依赖性上调 TNF-α 转录及蛋白水平[4]

生物活性

Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells.The ED50 for this effect is ≤0.4622 ng/mL, corresponding to a specific activity is ≥2.164×106 Unit/mg.

  • Measured by its ability to inhibit the proliferation of HT-29 cells. The ED50 for this effect is 0.1895 ng/mL, corresponding to a specific activity is 5.277×106 Unit/mg.
种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P01579 (Q24-Q166)

基因 ID
蛋白结构
N-term
IFN-gamma (Q24-Q166)
Accession # P01579
C-term
蛋白长度

Full Length of Mature Protein

中文名
干扰素-gamma 蛋白
同用名
Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG
氨基酸序列

QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ

分子量

20-25 & 16-17kDa

糖基化修饰
Yes
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 4% Mannitol, 2% Sucrose, 0.02% Tween80, pH 7.4 or PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IFN-gamma Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-gamma Protein, Human (HEK293)
目录号:
HY-P70610
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