IGF-I/IGF-1 蛋白, Mouse (N-His)

The IGF-I/IGF-1 protein is structurally similar to insulin and has potent growth-promoting activity. As a physiological regulator, it stimulates glucose transport and glycogen synthesis in osteoblasts, exceeding the efficacy of insulin at lower concentrations. IGF-I/IGF-1 Protein, Mouse (N-His) is the recombinant mouse-derived IGF-I/IGF-1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IGF-I/IGF-1 Protein, Mouse (N-His) is 70 a.a., with molecular weight of ~10 kDa.

IGF-I/IGF-1 蛋白在结构和功能上类似于胰岛素，具有显着增强的生长促进活性。作为一种生理调节剂，它可以控制成骨细胞中的 [1-14C]-2-脱氧-D-葡萄糖 (2DG) 转运和糖原合成，以明显低于胰岛素的浓度有效刺激骨源性成骨细胞 (PyMS) 中的葡萄糖转运。此外，IGF-I 可能有助于突触成熟和 Ca(2+) 依赖性胞吐作用，这对于嗅球中的气味感知至关重要。作为 IGF1R 的配体，它与 α 亚基结合，触发内在酪氨酸激酶活性的激活，从而导致 β 亚基中酪氨酸残基的自身磷酸化。这种启动引发了一系列下游信号传导事件，激活 PI3K-AKT/PKB 和 Ras-MAPK 通路。IGF-I 还与整合素（ITGAV:ITGB3 和 ITGA6:ITGB4）和 IGFR1 形成必需的三元复合物，以实现全面的 IGF1 信号传导，影响 IGFR1、MAPK3/ERK1、MAPK1/ERK2 和 AKT1 的磷酸化和激活。此外，它与 SH2D3C 同工型 2 相互作用，突出了其多样化的分子参与。

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells.The ED₅₀ for this effect is 3.972-6.159 ng/mL, corresponding to a specific activity is 1.623×10⁵ - 2.517×10⁵ units/mg.

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  Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells.The ED₅₀ for this effect is 3.972 ng/mL, corresponding to a specific activity is 2.517×10⁵ units/mg.

Mouse

E. coli

N-6*His

P05017 (G49-A118)

16000  [NCBI]

GPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKAA

Approximately 10 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.