IGF-I R 蛋白, Human (HEK293, His, solution)

IGF-I receptor (IGF1R) mediates insulin-like growth factor effects, binding strongly to IGF1. Activation triggers PI3K-AKT/PKB and Ras-MAPK pathways, influencing cell survival, protein synthesis, and proliferation. IGF1R also signals through JAK/STAT, inhibiting JNK activation. Hybrid receptors show variable binding to IGF1 and insulin. IGF-I R Protein, Human (HEK293, His, solution) is the recombinant human-derived IGF-I R protein, expressed by HEK293 , with C-His labeled tag. The total length of IGF-I R Protein, Human (HEK293, His, solution) is 902 a.a., with molecular weight of ~150 kDa.

IGF-I 受体 (IGF1R) 是一种受体酪氨酸激酶，在介导胰岛素样生长因子 1 (IGF1) 的作用中发挥着关键作用。它对 IGF1 表现出高亲和力，对 IGF2 和胰岛素 (INS) 表现出较低的亲和力。配体结合后，IGF1R 激活其激酶，导致受体自身磷酸化和各种底物的酪氨酸磷酸化，包括胰岛素受体底物 (IRS1/2)、Shc 和 14-3-3 蛋白。这会启动两条主要信号传导途径：PI3K-AKT/PKB 途径（抑制细胞凋亡并刺激蛋白质合成）和 Ras-MAPK 途径（促进细胞增殖）。磷酸化的 IRS1 可以激活 PI3K 通路，导致下游 AKT/PKB 激活，随后增强蛋白质合成和抗凋亡作用。同时，磷酸化的 IRS1 或 Shc 招募 Grb2/SOS 会激活 Ras-MAPK 通路。此外，IGF1R 通过 JAK/STAT 途径发出信号，可能有助于其转化活性。JNK激酶也可以被IGF1R激活，IGF1通过磷酸化和抑制MAP3K5/ASK1来抑制JNK激活。由 IGF1R 和 INSR 亚型组成的混合受体表现出不同的结合特征，表明对 IGF1 的高亲和力和对胰岛素的低亲和力。

1.Measured by its ability to bind IGF1 Protein, Human, Recombinant in functional ELISA.
2.Captured IGF1R on NTA Chip can bind IGF1 with an affinity constant of 0.1317 µM as determined in a SPR assay (Biacore T200).

Human

HEK293

C-His

P08069 (E31-N932)

3480  [NCBI]

MKSGSGGGSPTSLWGLLFLSAALSLWPTSGEICGPGIDIRNDYQQLKRLENCTVIEGYLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAVSNNYIVGNKPPKECGDLCPGTMEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSTCGKRACTENNECCHPECLGSCSAPDNDTACVACRHYYYAGVCVPACPPNTYRFEGWRCVDRDFCANILSAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDGYLYRHNYCSKDKIPIRKYADGTIDIEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERKRRDVMQVANTTMSSRSRNTTAADTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTGYEN

Approximately 150 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.