IL-10R beta 蛋白, Human (HEK293, His)

IL-10R beta protein is an IL10 cytokine surface receptor involved in IL10-mediated inflammation and immune regulation, as well as viral defense responses.IL-10R beta Protein, Human (HEK293, His) is expressed by HEK 293 cells and has a transmembrane region (W221-F242) with a 6*His tag at the C-terminus^[3].

IL-10受体复合物由四个跨膜链的异二聚体组成，其中两个形成IL-10Rα，其中两个形成IL-10Rβ。IL-10R beta 最初称为孤儿受体 CRF2-4，是活性白细胞介素 10 受体复合物的重要辅助亚基^[1]。
IL-10R beta 可与 IL 结合-10 通过 JAK-STAT 途径，IL-10 受体复合物的激活导致受体相关蛋白 TYK 和 JAK-1 磷酸化，并最终激活转录因子 STAT3、STAT1 和 STAT5，这些因子调节IL-10 响应基因（包括 c-myc、bcl-2 和 bcl-xL）的表达，启动信号转导^[2]。
IL-10R beta 在大多数细胞上表达细胞类型，也是激活五种 II 类细胞因子（IL-10、IL-22、IL-26、IL-28 和 IFNL1）所需的共享细胞表面受体，这些细胞因子在宿主防御、免疫调节中发挥关键作用。其中，IFNLR1/IL10R β二聚体是细胞因子配体 IFNL2 和 IFNL3 的受体，并介导其抗病毒活性^[3]。

IL-10R beta expression levels increase steadily during neuronal differentiation in human neuronal cells (NT2-N) and can be co-expressed with IL-28Rα, affecting the biological function of cells for IFN-λ^[4].

Immobilized Recombinant Human IL 28 R alpha /IFN lambda R1 at 5 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human IL-10R beta. The ED₅₀ for this effect is 9.714 μg/mL.

Human

HEK293

C-6*His

Q08334/NP_000619.3 (M20-S220)

3588  [NCBI]

MVPPPENVRMNSVNFKNILQWESPAFAKGNLTFTAQYLSYRIFQDKCMNTTLTECDFSSLSKYGDHTLRVRAEFADEHSDWVNITFCPVDDTIIGPPGMQVEVLADSLHMRFLAPKIENEYETWTMKNVYNSWTYNVQYWKNGTDEKFQITPQYDFEVLRNLEPWTTYCVQVRGFLPDRNKAGEWSEPVCEQTTHDETVPS

Approximately 48 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sung-Il Yoon, et al. Structure and mechanism of receptor sharing by the IL-10R2 common chain. Structure. 2010 May 12;18(5):638-48.  [Content Brief]

  [2]. J Shi, et al. IL10 inhibits starvation-induced autophagy in hypertrophic scar fibroblasts via cross talk between the IL10-IL10R-STAT3 and IL10-AKT-mTOR pathways. Cell Death Dis. 2016 Mar 10;7(3):e2133.  [Content Brief]

  [3]. Paul Sheppard, et al. IL-28, IL-29 and their class II cytokine receptor IL-28R. Nat Immunol. 2003 Jan;4(1):63-8.  [Content Brief]

  [4]. L. Zhou, et al. Activation of toll-like receptor-3 induces interferon-lambda expression in human neuronal cells. Neuroscience. 2009 Mar 17;159(2):629-37.