IL-11 蛋白, Cynomolgus

IL-11 protein is a potent cytokine that significantly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, leading to megakaryocyte maturation and platelet production. It also promotes hepatocyte proliferation in response to liver injury. IL-11 Protein, Cynomolgus is the recombinant cynomolgus-derived IL-11 protein, expressed by E. coli , with tag free. The total length of IL-11 Protein, Cynomolgus is 178 a.a., with molecular weight of ~20 KDa.

IL-11 蛋白是一种有效的细胞因子，在刺激造血干细胞和巨核细胞祖细胞的增殖、导致巨核细胞的成熟和随后的血小板产生方面发挥着至关重要的作用。此外，IL-11 蛋白可促进肝细胞增殖以应对肝损伤。IL-11 蛋白与其受体（由 IL6ST 和 IL11RA 组成）结合后，会触发信号级联反应，促进细胞增殖。该信号级联涉及细胞内蛋白激酶的激活和 STAT3 的磷酸化。IL-11 蛋白可参与两种类型的信号传导：“经典信号传导”通过与膜结合的 IL11RA 和 IL6ST 相互作用而发生，而“反式信号传导”则由 IL-11 和可溶性 IL11RA 与 IL6ST 的结合诱导。此外，IL-11 蛋白与 IL11RA 相互作用，形成与 IL6ST 相关的多聚体信号复合物。

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 4.216 ng/mL, corresponding to a specific activity is 2.371×10⁵ units/mg.

Cynomolgus

E. coli

Tag Free

XP_005595839.2/P20808 (P22-L199)

102118349

PGPPPGSPRASPDPRAELDSTVLLTRSLLEDTRQLTIQLKDKFPADGDHNLDSLPTLAMSAGALGALQLPSVLTRLRADLLSYLRHVQWLRRAMGSSLKTLEPELGTLQTRLDRLLRRLQLLMSRLALPQLPPDPPAPPLAPPSSTWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL

Approximately 20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.