IL-17A 蛋白, Mouse (E. coli)

IL-17A protein is an important effector cytokine that activates the NF-kappa-B and MAPkinase pathways through the IL17RA-IL17RC receptor complex to protect host tissues from microbial threats. As a key Th17 cytokine, IL-17A mediates neutrophil activation, chemotaxis, and contributes to germinal center formation. IL-17A Protein, Mouse (E. coli) is the recombinant mouse-derived IL-17A protein, expressed by E. coli , with tag free. The total length of IL-17A Protein, Mouse (E. coli) is 137 a.a., with molecular weight of ~11-15 kDa.

IL-17A 蛋白是一种效应细胞因子，在先天性和适应性免疫反应中发挥着关键作用，可保护宿主组织免受微生物威胁。它通过 IL17RA-IL17RC 受体复合物发出信号，激活 NF-kappa-B 和 MAPkinase 通路，协调免疫效应子的转录激活。作为标志性 Th17 细胞因子，IL-17A 介导中性粒细胞活化、趋化性，并有助于生发中心形成。它作为炎症回路的一部分，促进细菌清除，并且对于稳态和感染期间上皮屏障的完整性至关重要。IL-17A 增强抗病毒防御，并与 IL-17F 协同作用，诱导抗菌肽的产生。其同二聚体和异二聚体结构突出了其在免疫调节中的多样化相互作用。

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 2.547 ng/mL, corresponding to a specific activity is 3.926×10⁵ U/mg.

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  Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.The ED₅₀ for this effect is 2.547 ng/mL, corresponding to a specific activity is 3.926×105 U/mg.

Mouse

E. coli

Tag Free

Q62386 (T22-A158)

16171

TVKAAAIIPQSSACPNTEAKDFLQNVKVNLKVFNSLGAKVSSRRPSDYLNRSTSPWTLHRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVLKREPESCPFTFRVEKMLVGVGCTCVASIVRQAA

Approximately 11-15 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.