1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors
  4. IL-17
  5. IL-17F
  6. IL-17F 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

IL-17F 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

目录号: HY-P78160
产品使用指南

IL-17F 是一种炎性同型二聚体细胞因子,可诱导许多促炎细胞因子和趋化因子。IL-17F 也能诱导抗菌肽。IL-17F 在结肠癌发展中起保护作用,可用于自身免疫性疾病、感染和癌症的研究。IL-17F 蛋白, Mouse (Biotinylated, HEK293, His-Avi) 是一种生物素化的重组小鼠 IL-17F 蛋白,在 HEK293 细胞中表达,C 端带有 His 和Avi 标签,由 133 个氨基酸组成 (R29-A161)。

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100 μg 询问价格 & 货期

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描述

IL-17F is an inflammatory homodimeric cytokine that induces many proinflammatory cytokines and chemokines. IL-17F also induces antimicrobial peptides. IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer[1][2]. IL-17F Protein, Mouse (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant mouse IL-17F protein with His and Avi tags at the C-terminus and is expressed in HEK293 cells. It consists of 133 amino acids (R29-A161).

研究背景

Interleukin-17F (IL-17F) belongs to the IL-17 cytokine family. IL-17F is expressed in activated CD4 T cells, activated monocytes, basophils and mast cells. IL-17F can be produced by differentiated TH17 cells, lamina propria T cells, memory CD4+ T cells, γδ T cells and NKT cells[1].
The mouse IL-17F shares 55.90% amino acid sequence identity with human and 86.34% identity with rat.
IL-17F is an inflammatory cytokine that induces many proinflammatory cytokines and chemokines, including TGF-β, IL-2, ICAM1, GM-CSF, CCL2, CCL7, TSLP, MMP13, IL-6 and CXCL1. IL-17F also induces antimicrobial peptides including hBD-2, S100A7, S100A8 and S100A9 with IL-22 and can synergize with IL-23 in human eosinophils to promote the production of IL-1β and IL-6. IL-17F is a homodimeric cytokine. IL-17F shares the most similarities with IL-17A (50% homology) and can be produced as an IL-17AF heterodimer. IL-17A, IL-17F and IL-17A/F use the same receptor complex: IL-17RA and IL-17RC heterodimer. They trigger qualitatively similar signaling pathways, and IL-17F exhibits the lowest biological activity. IL-17F shows about 100–1000 times lower affinity to the IL-17RA subunit than IL-17A, and does not compete with IL-17A binding to IL-17RA[1][2].
IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer[1][3][4].

体外研究
(In Vitro)

IL-17F (100 ng/mL; overnight) induces proinflammatory cytokines and chemokines in vitro via IL-17RA, TRAF6, and Act1 in mouse embryonic fibroblasts (MEFs)[5].

体内研究
(In Vivo)

IL-17F (0.5 μg/mouse; i.p.; once) results in the recruitment of neutrophils within 4 h in mice[5].

生物活性

Immobilized Mouse IL-17RA hFc at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Mouse IL-17F His with the EC50 of 2.9 μg/mL determined by ELISA.

种属

Mouse

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q7TNI7 (R29-A161)

基因 ID

257630  [NCBI]

中文名
重组小鼠白细胞介素 17F 蛋白 (Biotinylated, HEK293, His-Avi)
同用名
Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1
分子量

30-35 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Solution.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

IL-17F Protein, Mouse (Biotinylated, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-17F Protein, Mouse (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78160
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