1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-18
  5. IL-18 蛋白, Dog (His)

IL-18 蛋白, Dog (His)

目录号: HY-P71657
产品使用指南

IL-18 蛋白是一种促炎细胞因子,对于上皮屏障修复和免疫调节至关重要,尤其是在 Th1 和 NK 细胞反应中。IL18R1 和 IL18RAP 结合形成信号三元复合物,激活 NF-κ-B 并诱导炎症介质。IL-18 蛋白, Dog (His) 是重组的 IL-18 蛋白,由 E. coli 表达,带有 N-His 标签。IL-18 蛋白, Dog (His) 全长 157 个氨基酸,分子量约为 ~22.0 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

IL-18 protein is a pro-inflammatory cytokine that is critical for epithelial barrier repair and immune regulation, especially in Th1 and NK cell responses. IL18R1 and IL18RAP combine to form a signaling ternary complex that activates NF-kappa-B and induces inflammatory mediators. IL-18 Protein, Dog (His) is the recombinant dog-derived IL-18 protein, expressed by E. coli , with N-His labeled tag. The total length of IL-18 Protein, Dog (His) is 157 a.a., with molecular weight of ~22.0 kDa.

研究背景

IL-18 蛋白是一种促炎细胞因子,在上皮屏障修复和调节免疫反应中发挥着至关重要的作用,特别是在极化辅助性 T 1 (Th1) 细胞和自然杀伤 (NK) 细胞免疫反应中。它与 IL18R1 和 IL18RAP 结合后形成信号三元复合物,激活 NF-kappa-B 并触发炎症介质的合成。IL-18 还与 IL-12/IL-12 协同作用,诱导 Th1 细胞和 NK 细胞合成 IFNG。此外,IL-18 参与细胞焦亡下游的炎症转导,其成熟形式通过gasdermin-D (GSDMD) 孔选择性释放到细胞外环境中。它与质膜上的配体结合受体亚基IL18R1和信号受体亚基IL18RAP形成三元复合物,其与货物受体TMED10的相互作用介导从细胞质易位至ERGIC(内质网-高尔基中间室)进行分泌。

种属

Dog

表达系统

E. coli

标签

N-His

蛋白编号

Q9XSR0 (37Y-193S)

基因 ID

403796  [NCBI]

蛋白结构
N-term
His
IL-18 (37Y-193S)
Accession # Q9XSR0
C-term
同用名
IL18; IGIF; Interleukin-18; IL-18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
氨基酸序列

YFGKLEPKLSIIRNLNDQVLFVNEGNQPVFEDMPDSDCTDNAPHTIFIIYMYKDSLTRGLAVTISVKYKTMSTLSCKNKTISFQKMSPPDSINDEGNDIIFFQRSVPGHDDKIQFESSLYKGHFLACKKENDLFKLILKDKDENGDKSIMFTVQNKS

分子量

Approximately 22.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IL-18 Protein, Dog (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
IL-18 Protein, Dog (His)
目录号:
HY-P71657
需求量: