IL-1F10/IL-38 蛋白, Human (P.pastoris, His)

IL-1F10/IL-38 protein is an immunomodulatory cytokine that indirectly regulates cytokine production. It reduces IL22 and IL17A production in T cells exposed to heat-killed Candida albicans and inhibits IL36G-induced IL8 production in peripheral blood mononuclear cells. IL-1F10/IL-38 Protein, Human (P.pastoris, His) is the recombinant human-derived IL-1F10/IL-38 protein, expressed by P. pastoris , with C-His labeled tag. The total length of IL-1F10/IL-38 Protein, Human (P.pastoris, His) is 152 a.a., with molecular weight of 20-23 KDa.

IL-1F10/IL-38 蛋白是一种免疫调节细胞因子，不直接诱导细胞因子的产生。相反，在暴露于热灭活的白色念珠菌后，它通过减少 T 细胞中 IL22 和 IL17A 的产生来发挥调节作用。此外，它还能抑制外周血单核细胞中 IL36G 诱导的 IL8 产生。有趣的是，IL-1F10/IL-38 蛋白可增强细菌脂多糖 (LPS) 刺激的树突状细胞中 IL6 的产生。它作为 IL-36R/IL1RL2 的配体，并与货物受体 TMED10 相互作用，促进从细胞质易位至 ERGIC（内质网-高尔基中间室）和随后的分泌。

Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 μM as determined in SPR assay (Biacore T200).

Human

P. pastoris

C-His

Q8WWZ1 (M1-W152)

84639  [NCBI]

20-23 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.