IL-21 蛋白, Canine

IL-21 is a potent immunoregulatory cytokine that orchestrates the delicate interplay between innate and adaptive immunity. It drives the production of IgG(1) and IgG(3) in B cells, which are critical for humoral immune responses, and plays a key role in T follicular helper (Tfh) cell generation and germinal center formation. IL-21 Protein, Canine is the recombinant canine-derived IL-21 protein, expressed by E. coli , with tag free. The total length of IL-21 Protein, Canine is 129 a.a., with molecular weight of ~15 kDa.

IL-21 是一种具有强大免疫调节活性的细胞因子，在先天免疫和适应性免疫之间的微妙相互作用中发挥着中心协调作用。IL-21 展现出其多方面的影响，可刺激 B 细胞中 IgG(1) 和 IgG(3) 的产生，强调其在形成体液免疫反应中的关键作用。细胞因子与滤泡辅助 T (Tfh) 细胞的生成和维持以及生发中心的动态形成密切相关。IL-21 与 IL6 协同作用，在指导 Tfh 细胞的早期发育中发挥着关键作用，事实证明，IL-21 对于在急性病毒感染期间建立有效的抗体反应是不可或缺的。IL-21 扩大其调节范围，与 IL15 协同作用，可能有助于自然杀伤 (NK) 细胞的增殖和成熟。此外，IL-21 发挥调节作用，控制成熟 B 细胞和 T 细胞响应激活刺激的增殖。它与 IL15 和 IL18 协同作用，刺激 T 细胞和 NK 细胞中干扰素 γ 的产生，为其复杂的调节功能增添了层次。值得注意的是，在 T 细胞介导的免疫反应过程中，IL-21 作为一种潜在的抑制剂出现，抑制树突状细胞的激活和成熟，进一步凸显其在免疫调节中的微妙作用。

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The ED₅₀ for this effect is typically 0.4-2 ng/mL.

Canine

E. coli

Tag Free

Q6L7I9 (H18-S146)

442935  [NCBI]

Approximately 15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.