1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-5
  5. IL-5 蛋白, Pig (His)

IL-5 蛋白, Pig (His)

目录号: HY-P71630
产品使用指南

IL-5 蛋白由 T 淋巴细胞和 NK 细胞表达,对于嗜酸性粒细胞的存活、分化和趋化性至关重要。它还影响 B 细胞,诱导免疫球蛋白的产生和生长。IL-5 蛋白通过 IL5RA 和 CSF2RB 亚基激活激酶 LYN、SYK 和 JAK2,通过 RAS-MAPK 和 JAK-STAT5 途径发出信号。它以二硫键连接的同二聚体形式存在。IL-5 蛋白, Pig (His) 是重组的 IL-5 蛋白,由 E. coli 表达,带有 N-His 标签。IL-5 蛋白, Pig (His) 全长 113 个氨基酸,分子量约为 ~17.0 kDa。

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重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

IL-5 Protein, expressed by T-lymphocytes and NK cells, is vital for eosinophil survival, differentiation, and chemotaxis. It also affects B-cells, inducing immunoglobulin production and growth. IL-5 Protein activates kinases LYN, SYK, and JAK2 through IL5RA and CSF2RB subunits, signaling via RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer. IL-5 Protein, Pig (His) is the recombinant pig-derived IL-5 protein, expressed by E. coli , with N-His labeled tag. The total length of IL-5 Protein, Pig (His) is 113 a.a., with molecular weight of ~17.0 kDa.

研究背景

IL-5 蛋白是一种同型二聚体细胞因子,主要由 T 淋巴细胞和 NK 细胞表达,在嗜酸性粒细胞的存活、分化和趋化性中发挥着至关重要的作用。它还作用于激活的和静止的 B 细胞,诱导免疫球蛋白的产生、生长和分化。从机制上讲,IL-5蛋白通过IL5RA亚基和细胞因子受体共同亚基β/CSF2RB组成的受体发挥其生物学作用,导致LYN、SYK和JAK2等多种激酶的激活。这些激活的激酶通过 RAS-MAPK 和 JAK-STAT5 途径传播信号。IL-5 蛋白以二硫键连接的同二聚体形式存在,并与 IL5RA 和 CSF2RB 亚基相互作用。

种属

Pig

表达系统

E. coli

标签

N-His

蛋白编号

Q9MYM5 (22V-134S)

基因 ID
蛋白结构
N-term
His
IL-5 (22V-134S)
Accession # Q9MYM5
C-term
同用名
IL5; Interleukin-5; IL-5; Eosinophil differentiation factor; T-cell replacing factor; TRF
氨基酸序列

VENTMNRLVAETLTLLSIHRTLLIGDGNLMISTPVHTNHQLCIEEVFQGIDTLKNQTARGDAVEKLFQNLSLIKEYIDRQKKNCGGERWRVTQFLDYLQVFLGVINTEWTMES

分子量

Approximately 17.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IL-5 Protein, Pig (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-5 Protein, Pig (His)
目录号:
HY-P71630
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