1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors
  4. IL-5 Receptor
  5. IL-5 Receptor α
  6. IL-5R alpha 蛋白, Human (Biotinylated, HEK293, His-Avi)

IL-5R alpha 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78162
COA 产品使用指南

IL-5R alpha 蛋白是一种细胞因子受体,在嗜酸性粒细胞、嗜碱性粒细胞及B 细胞上表达,可参与炎症及免疫调控。重组人 IL-5R alpha 蛋白 (Biotinylated, HEK293, His-Avi) 是由 HEK 293 细胞表达的,是跨膜蛋白 (F343-C362),带有 His-Avi 标签。

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描述

IL-5R alpha protein is a cytokine receptor that is expressed on eosinophils, basophils and B cells and is involved in inflammation and immune regulation.IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) is expressed by HEK293 cells and is a transmembrane protein (F343-C362) with His-Avi tags[1][3].

研究背景

IL-5R alpha is a type I cytokine receptor consisting of two distinct polypeptide chains, alpha and beta. alpha chain is a membrane-penetrating glycoprotein that specifically binds IL-5 and beta chain converts low affinity IL-5R to high affinity IL-5R, forming a heterodimeric receptor complex[1].
IL-5R alpha can induce the activation of multiple intracellular signalling pathways, including JAK/STAT, RAF/MAP, Ras-Raf-ERK and phosphatidylinositol 3-kinase when its binds with IL-5[2].
IL-5R alpha is expressed on eosinophils, basophils and B cells and affects cell survival, apoptosis, differentiation and chemotaxis[3].

生物活性

Measured by its binding ability in a functional ELISA. When immobilized Human IL-5 His at 0.5 μg/mL (100μL/Well) , can bind biotinylated Human IL-5 R alpha His and the EC50 is 0.12 ug/mL.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q01344 (D21-E335)

基因 ID

3568  [NCBI]

中文名
重组人 IL-5R alpha 蛋白 (His-Avi)
同用名
CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560
分子量

Approximately 38.7 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi) 相关分类

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-5R alpha Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78162
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