1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors Neurotrophic Factors
  4. IL-6
  5. IL-6 蛋白, Mouse (CHO, His)

IL-6 蛋白, Mouse (CHO, His)

目录号: HY-P7378
COA 产品使用指南

IL-6 蛋白, Mouse (CHO, His)是带有 His 标签的 白细胞介素6, 是具有多功能的细胞因子,可调节多种免疫和炎症反应。

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规格 价格 是否有货 数量
10 μg ¥1980 In-stock
50 μg ¥3980 In-stock
100 μg   询价  

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  • 生物活性

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  • 产品资料

  • 参考文献

描述

IL-6 Protein, Mouse (CHO, His) is a multifunctional cytokine that can regulate various immune and inflammatory responses.

研究背景

白介素 6 (IL-6) 是肝脏急性时相反应最重要的介质之一[1]。白细胞介素 6 (IL-6) 在感染和组织损伤时迅速而短暂地产生,通过刺激急性期反应、造血和免疫反应来促进宿主防御。IL-6 促进初始 CD4+ T 细胞的特异性分化,从而在连接先天免疫反应和获得性免疫反应中发挥重要作用。IL-6 与转化生长因子 (TGF)-β 结合,对于幼稚 CD4+ T 细胞向 Th17 分化是必不可少的,但 IL-6 也会抑制 TGF-β 诱导的 Treg 分化[1]

生物活性

The ED50 is <3 pg/mL as measured by 7TD1 cells.

种属

Mouse

表达系统

CHO

标签

C-His

蛋白编号

P08505 (F25-T211)

基因 ID
蛋白结构
N-term
IL-6 (F25-T211)
Accession # P08505
His
C-term
中文名
重组小鼠白细胞介素- 6, His (CHO 表达)
同用名
rMuIL-6, His; B cell differentiation factor; IL6
氨基酸序列

FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQTHHHHHH

分子量

24-26 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-6 Protein, Mouse (CHO, His)
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HY-P7378
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