1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-7
  5. IL-7 蛋白, Human (CHO, His)

IL-7 蛋白, Human (CHO, His)

目录号: HY-P72792
COA 产品使用指南

IL-7蛋白是一种重要的造血细胞因子,对于T细胞和B细胞的发育、扩增和生存、调节成熟淋巴细胞群和维持淋巴稳态至关重要。IL-7 与 IL7RA 和 CSF2RG 亚基相互作用,激活激酶(包括 JAK1 或 JAK3),并启动信号级联,例如 PI3K/Akt/mTOR 或 JAK-STAT5 通路。IL-7 蛋白, Human (CHO, His) 是重组的 IL-7 蛋白,由 CHO 表达,带有 C-His 标签。IL-7 蛋白, Human (CHO, His) 全长 152 个氨基酸,分子量为 24-30 kDa。

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规格 价格 是否有货 数量
10 μg ¥2320 In-stock
50 μg ¥6500 In-stock
100 μg   询价  

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描述

IL-7 protein is an important hematopoietic cytokine that is essential for the development, expansion and survival of T cells and B cells, regulating mature lymphocyte populations and maintaining lymphatic homeostasis. IL-7 interacts with IL7RA and CSF2RG subunits, activates kinases, including JAK1 or JAK3, and initiates signaling cascades, such as the PI3K/Akt/mTOR or JAK-STAT5 pathway. IL-7 Protein, Human (CHO, His) is the recombinant human-derived IL-7 protein, expressed by CHO , with C-His labeled tag. The total length of IL-7 Protein, Human (CHO, His) is 152 a.a., with molecular weight of 24-30 kDa.

研究背景

IL-7蛋白作为重要的造血细胞因子,在幼稚T细胞、记忆T细胞以及B细胞的发育、扩增和存活中发挥着不可或缺的作用,从而调节成熟淋巴细胞的数量并维持淋巴稳态。其生物学效应是通过由IL7RA亚基和细胞因子受体共同亚基γ/CSF2RG组成的受体来执行的。与受体结合后,IL-7 会激活各种激酶,包括 JAK1JAK3,具体取决于细胞类型。这种激活导致信号通过多个下游途径传播,例如 PI3K/Akt/mTOR 或 JAK-STAT5 途径。IL-7 与 IL7R 和 CSF2RG 的相互作用凸显了其在协调对免疫细胞发育和功能至关重要的多种信号级联中的关键作用。

生物活性

The cell proliferation assay using 2E8 cells has an ED50 value of less than 2 ng/ml.

种属

Human

表达系统

CHO

标签

C-His

蛋白编号

P13232 (D26-H177)

基因 ID
蛋白结构
N-term
IL-7 (D26-H177)
Accession # P13232
His
C-term
同用名
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
氨基酸序列

DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH

分子量

24-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<0.2 EU/μg; determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-7 Protein, Human (CHO, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-7 Protein, Human (CHO, His)
目录号:
HY-P72792
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